Evidence map›Paper›PMID 26004439›Full record

ArticleStructure (London, England : 1993)2015

Molecular mechanism of resolving trinucleotide repeat hairpin by helicases.

Yupeng Qiu, Hengyao Niu, Lela Vukovic, Patrick Sung, Sua Myong

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Structure (London, England : 1993), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.0field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 7 citations in OpenAlex.

  1. Dynamic Assemblies in Genome Maintenance.Advances in experimental medicine and biology · 2026
    Review
  2. Review
  3. Parity-dependent hairpin configurations of repetitive DNA sequence promote slippage associated with DNA expansion.Proceedings of the National Academy of Sciences of the United States of America · 2017
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Yupeng QiuBioengineering Department, University of Illinois, 1304 West Springfield Avenue, Urbana, IL 61801, USA.
Hengyao NiuDepartment of Molecular Biophysics and Biochemistry, Yale University, 333 Cedar Street, PO Box 208024, New Haven, CT 06520, USA.
Lela VukovicDepartment of Physics, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA; Physics Frontier Center (Center of Physics for Living Cells), University of Illinois, 1110 West Green Street, Urbana, IL 61801, USA.
Patrick SungDepartment of Molecular Biophysics and Biochemistry, Yale University, 333 Cedar Street, PO Box 208024, New Haven, CT 06520, USA.
Sua MyongBioengineering Department, University of Illinois, 1304 West Springfield Avenue, Urbana, IL 61801, USA; Institute for Genomic Biology, University of Illinois, 1206 West Gregory Street, Urbana, IL 61801, USA; Physics Frontier Center (Center of Physics for Living Cells), University of Illinois, 1110 West Green Street, Urbana, IL 61801, USA; Biophysics and Computational Biology, University of Illinois, 1110 West Green Street, Urbana, IL 61801, USA. Electronic address: smyong@illinois.edu.
University of Illinois Urbana-Champaign · USYale University · US

Funding

Quantitative Stepwise Analysis of RNA InterferenceDP2GM105453 · NIGMS · UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN · PI MYONG, SUA · 2012 to 2012
$2.4M
NCCDPHP CDC HHS 1 DP2 GM105453 ANIGMS NIH HHS DP2 GM105453
6 · The paper itself

Abstract

Trinucleotide repeat (TNR) expansion is the root cause for many known congenital neurological and muscular disorders in human including Huntington's disease, fragile X syndrome, and Friedreich's ataxia. The stable secondary hairpin structures formed by TNR may trigger fork stalling during replication, causing DNA polymerase slippage and TNR expansion. Srs2 and Sgs1 are two helicases in yeast that resolve TNR hairpins during DNA replication and prevent genome expansion. Using single-molecule fluorescence, we investigated the unwinding mechanism by which Srs2 and Sgs1 resolves TNR hairpin and compared it with unwinding of duplex DNA. While Sgs1 unwinds both structures indiscriminately, Srs2 displays repetitive unfolding of TNR hairpin without fully unwinding it. Such activity of Srs2 shows dependence on the folding strength and the total length of TNR hairpin. Our results reveal a disparate molecular mechanism of Srs2 and Sgs1 that may contribute differently to efficient resolving of the TNR hairpin.

Indexed as

Models, MolecularProtein UnfoldingDNA HelicasesFluorescenceFluorescence Resonance Energy TransferInverted Repeat SequencesProtein ConformationRecQ HelicasesSaccharomyces cerevisiae ProteinsTrinucleotide RepeatsDNA HelicasesRecQ HelicasesSaccharomyces cerevisiae ProteinsSGS1 protein, S cerevisiaeSRS2 protein, S cerevisiae

Identifiers

PMID26004439
PMCPMC4456222
OpenAlexW1532780122

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.