Evidence map›Paper›PMID 26004237›Full record

ArticleBiomaterials2015

Engineered composite tissue as a bioartificial limb graft.

Bernhard J Jank, Linjie Xiong, Philipp T Moser, Jacques P Guyette, Xi Ren, Curtis L Cetrulo, David A Leonard, Leopoldo Fernandez, Shawn P Fagan, Harald C Ott

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
51citing papers in PubMed, 2 pooled it
10.0field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

51 citing papers in PubMed, 2 syntheses or guidelines pooled it, 116 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
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  16. Three-dimensionalNeural regeneration research · 2022
    Review
  17. Review
  18. Decellularized vascularized bone grafts: A preliminaryFrontiers in bioengineering and biotechnology · 2022
    Article
  19. Regenerative medicine technologies applied to transplant medicine. An update.Frontiers in bioengineering and biotechnology · 2022
    Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Bernhard J JankCenter for Regenerative Medicine, Massachusetts General Hospital, USA; Harvard Medical School, Boston, MA, USA.
Linjie XiongCenter for Regenerative Medicine, Massachusetts General Hospital, USA.
Philipp T MoserCenter for Regenerative Medicine, Massachusetts General Hospital, USA; Harvard Medical School, Boston, MA, USA.
Jacques P GuyetteCenter for Regenerative Medicine, Massachusetts General Hospital, USA; Harvard Medical School, Boston, MA, USA.
Xi RenCenter for Regenerative Medicine, Massachusetts General Hospital, USA; Harvard Medical School, Boston, MA, USA.
Curtis L CetruloHarvard Medical School, Boston, MA, USA; Transplantation Biology Research Center, Department of Surgery, Massachusetts General Hospital, USA.
David A LeonardHarvard Medical School, Boston, MA, USA; Transplantation Biology Research Center, Department of Surgery, Massachusetts General Hospital, USA.
Leopoldo FernandezCenter for Regenerative Medicine, Massachusetts General Hospital, USA.
Shawn P FaganMassachusetts General Hospital, Division of Burn Surgery, Harvard Medical School, USA.
Harald C OttDivision of Thoracic Surgery, Department of Surgery, Massachusetts General Hospital, USA; Harvard Medical School, Boston, MA, USA. Electronic address: hott@partners.org.
Harvard University · USMassachusetts General Hospital · US

Funding

Organ engineering based on perfusion decellularized matrixDP2OD008749 · OD · MASSACHUSETTS GENERAL HOSPITAL · PI OTT, HARALD C · 2011 to 2011
$2.6M
NIH HHS DP2 OD008749
6 · The paper itself

Abstract

The loss of an extremity is a disastrous injury with tremendous impact on a patient's life. Current mechanical prostheses are technically highly sophisticated, but only partially replace physiologic function and aesthetic appearance. As a biologic alternative, approximately 70 patients have undergone allogeneic hand transplantation to date worldwide. While outcomes are favorable, risks and side effects of transplantation and long-term immunosuppression pose a significant ethical dilemma. An autologous, bio-artificial graft based on native extracellular matrix and patient derived cells could be produced on demand and would not require immunosuppression after transplantation. To create such a graft, we decellularized rat and primate forearms by detergent perfusion and yielded acellular scaffolds with preserved composite architecture. We then repopulated muscle and vasculature with cells of appropriate phenotypes, and matured the composite tissue in a perfusion bioreactor under electrical stimulation in vitro. After confirmation of composite tissue formation, we transplanted the resulting bio-composite grafts to confirm perfusion in vivo.

Indexed as

Artificial LimbsBioartificial OrgansTissue ScaffoldsAnimalsCell DifferentiationCell-Free SystemCells, CulturedEquipment Failure AnalysisExtracellular MatrixMaleMuscle, SkeletalProsthesis DesignRatsRats, Sprague-DawleyStem CellsTissue EngineeringBioprosthesisBone graftMechanical propertiesMuscle

Identifiers

PMID26004237
PMCPMC4568187
OpenAlexW317501330

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.