ArticleSurgery2015
Preservation solution impacts physiologic function and cellular viability of human saphenous vein graft.
Article in Surgery, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it, 44 citations in OpenAlex.
- Pooled it
- Vein graft failure: Pathophysiology, detection, prevention and emerging therapeutic strategies.Pharmacological reviews · 2026Review
- Prospective Study of Recipient Human Leukocyte Antigen (HLA) Alloimmunization Following the Use of Cold-Stored Saphenous Vein Allografts in Vascular Surgery.Journal of clinical medicine · 2025Article
- Intravascular delivery of an MK2 inhibitory peptide to prevent restenosis after angioplasty.Biomaterials · 2025Article
- Effect of preservation solution and distension pressure on saphenous vein's endothelium.Interactive cardiovascular and thoracic surgery · 2022Article
- Effect of different storage solutions on oxidative stress in human saphenous vein grafts.Journal of cardiothoracic surgery · 2022Article
- Current intraoperative storage and handling practices of autologous bypass conduit: A survey of the royal australasian college of surgeons.Frontiers in surgery · 2022Article
- A Novel Endothelial Damage Inhibitor Reduces Oxidative Stress and Improves Cellular Integrity in Radial Artery Grafts for Coronary Artery Bypass.Frontiers in cardiovascular medicine · 2021Article
- A cell permeant phosphopeptide mimetic of Niban inhibits p38 MAPK and restores endothelial function after injury.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- Do storage solutions protect endothelial function of arterialized vein graft in an experimental rat model?Journal of cardiothoracic surgery · 2020Article
- A novel endothelial damage inhibitor for the treatment of vascular conduits in coronary artery bypass grafting: protocol and rationale for the European, multicentre, prospective, observational DuraGraft registry.Journal of cardiothoracic surgery · 2019Observational
- Article
- Clinical factors that influence the cellular responses of saphenous veins used for arterial bypass.Journal of vascular surgery · 2018Observational
- 0.9% NaCl (Normal Saline) - Perhaps not so normal after all?Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis · 2018Review
- P2X7R antagonism after subfailure overstretch injury of blood vessels reverses vasomotor dysfunction and prevents apoptosis.Purinergic signalling · 2017Article
- Limiting Injury During Saphenous Vein Graft Preparation For Coronary Arterial Bypass Prevents Metabolic Decompensation.Scientific reports · 2017Article
- Adenosine triphosphate as a molecular mediator of the vascular response to injury.The Journal of surgical research · 2017Article
- Increased Contractile Function of Human Saphenous Vein Grafts Harvested by "No-Touch" Technique.Frontiers in physiology · 2017Article
- Vascular surgical stretch injury leads to activation of P2X7 receptors and impaired endothelial function.PloS one · 2017Article
- Traditional graft preparation decreases physiologic responses, diminishes viscoelasticity, and reduces cellular viability of the conduit: A porcine saphenous vein model.Vascular medicine (London, England) · 2016Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
introductionRecent clinical data suggest intraoperative preservation of human saphenous vein (HSV) in normal saline is associated with vein graft failure. We evaluated the influence of several preservation media on acute physiologic function and cellular viability of HSV conduit.
methodsUnprepared (UP) HSV obtained from coronary artery bypass graft patients was characterized on a muscle bath after 2-hour storage in 6 solutions: Plasma-Lyte A, 0.9% NaCl (normal saline), University of Wisconsin solution, Celsior solution, autologous whole blood, or glutathione-ascorbic acid L-arginine (GALA) solution. Vascular smooth muscle contractility was assessed after exposure to depolarizing KCl and phenylephrine. The relaxation of phenylephrine-precontracted HSV to sodium nitroprusside and carbachol (endothelial-independent and -dependent relaxation, respectively) was also assessed. Cellular viability was determined via the methyl thiazolyl tetrazolium (MTT) assay. Rat aortae were used to assess the effect of pH during graft preservation on endothelial-dependent relaxation.
resultsPreservation of HSV in normal saline and autologous whole blood impaired contractile responses to KCl relative to UP tissues, whereas preservation in University of Wisconsin solution and Celsior solution enhanced contractile responses (P < .05). Relative to UP tissues, responses to phenylephrine were decreased with preservation in normal saline, whereas preservation in University of Wisconsin solution, Celsior solution, and GALA all potentiated these responses (P < .05). Only preservation in normal saline impaired endothelial-independent relaxation (P = .005). Preservation in Plasma-Lyte A (P = .02), normal saline (P = .002), and University of Wisconsin solution (P = .02) impaired endothelial-dependent relaxation. Normal saline preservation decreased MTT viability index relative to UP tissues (0.02 ± 0.002 mg(-1)0.5 mL(-1) vs 0.033 ± 0.005 mg(-1)0.5 mL(-1); P = .03). Endothelial function was impaired by acidic pH in rat aorta.
conclusionPreservation of HSV in normal saline causes graft injury leading to impaired physiologic function and decreased viability of the HSV. This harm is mitigated by the use of buffered salt solutions as preservation media.
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Registered trials
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