ArticleMolecular & cellular proteomics : MCP2015
Bisecting Galactose as a Feature of N-Glycans of Wild-type and Mutant Caenorhabditis elegans.
Article in Molecular & cellular proteomics : MCP, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 37 citations in OpenAlex.
- Integrative Quantitative Proteomics and N-Glycoproteomics Reveal Age-Processing of N-Glycosylation in Caenorhabditis elegans.Proteomics · 2026Article
- Structural and functional characterization of the newly identified Photorhabdus laumondii tumor necrosis factor-like lectin.The FEBS journal · 2026Article
- Article
- Distinct clusters of human pain gene orthologs in Caenorhabditis elegans regulate thermo-nociceptive sensitivity and plasticity.Genetics · 2023Article
- Increasing Complexity of the N-Glycome During Caenorhabditis Development.Molecular & cellular proteomics : MCP · 2023Article
- Negative-mode mass spectrometry in the analysis of invertebrate, fungal, and protist N-glycans.Mass spectrometry reviews · 2022Review
- Towards understanding the extensive diversity of protein N-glycan structures in eukaryotes.Biological reviews of the Cambridge Philosophical Society · 2022Review
- Comparisons of N-glycans across invertebrate phyla.Parasitology · 2019Review
- Structures and functions of invertebrate glycosylation.Open biology · 2019Review
- Highly modified and immunoactive N-glycans of the canine heartworm.Nature communications · 2019Article
- N-glycomic Complexity in Anatomical Simplicity:Frontiers in molecular biosciences · 2019Review
- Ablation of N-acetylglucosaminyltransferases in Caenorhabditis induces expression of unusual intersected and bisected N-glycans.Biochimica et biophysica acta. General subjects · 2018Article
- The parasitic nematode Oesophagostomum dentatum synthesizes unusual glycosaminoglycan-like O-glycans.Glycobiology · 2018Article
- Identification of multiple isomeric core chitobiose-modified high-mannose and paucimannoseThe Journal of biological chemistry · 2018Article
- A comprehensive Caenorhabditis elegans N-glycan shotgun array.Glycobiology · 2018Article
- Core Richness of N-Glycans of Caenorhabditis elegans: A Case Study on Chemical and Enzymatic Release.Analytical chemistry · 2018Article
- Article
- Gender and developmental specific N-glycomes of the porcine parasite Oesophagostomum dentatum.Biochimica et biophysica acta. General subjects · 2017Article
- Analysis of Invertebrate and Protist N-Glycans.Methods in molecular biology (Clifton, N.J.) · 2017Article
- Article
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
The N-glycosylation of the model nematode Caenorhabditis elegans has proven to be highly variable and rather complex; it is an example to contradict the existing impression that "simple" organisms possess also a rather simple glycomic capacity. In previous studies in a number of laboratories, N-glycans with up to four fucose residues have been detected. However, although the linkage of three fucose residues to the N,N'-diacetylchitobiosyl core has been proven by structural and enzymatic analyses, the nature of the fourth fucose has remained uncertain. By constructing a triple mutant with deletions in the three genes responsible for core fucosylation (fut-1, fut-6 and fut-8), we have produced a nematode strain lacking products of these enzymes, but still retaining maximally one fucose residue on its N-glycans. Using mass spectrometry and HPLC in conjunction with chemical and enzymatic treatments as well as NMR, we examined a set of α-mannosidase-resistant N-glycans. Within this glycomic subpool, we can reveal that the core β-mannose can be trisubstituted and so carries not only the ubiquitous α1,3- and α1,6-mannose residues, but also a "bisecting" β-galactose, which is substoichiometrically modified with fucose or methylfucose. In addition, the α1,3-mannose can also be α-galactosylated. Our data, showing the presence of novel N-glycan modifications, will enable more targeted studies to understand the biological functions and interactions of nematode glycans.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.