ArticleeLife2015
The homo-oligomerisation of both Sas-6 and Ana2 is required for efficient centriole assembly in flies.
Article in eLife, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed.
- Centrioles generate two scaffolds with distinct biophysical properties to build mitotic centrosomes.Science advances · 2025Article
- TheCells · 2025Article
- The core spindle pole body scaffold Ppc89 links the pericentrin orthologue Pcp1 to the fission yeast spindle pole body via an evolutionarily conserved interface.Molecular biology of the cell · 2024Article
- Ana1/CEP295 is an essential player in the centrosome maintenance program regulated by Polo kinase and the PCM.EMBO reports · 2024Article
- Molecular Mechanism of STIL Coiled-Coil Domain Oligomerization.International journal of molecular sciences · 2023Article
- Centriole growth is limited by the Cdk/Cyclin-dependent phosphorylation of Ana2/STIL.The Journal of cell biology · 2022Article
- Structures of SAS-6 coiled coil hold implications for the polarity of the centriolar cartwheel.Structure (London, England : 1993) · 2022Article
- Review
- Daughter centrioles assemble preferentially towards the nuclear envelope inOpen biology · 2022Article
- Kinetic and structural roles for the surface in guiding SAS-6 self-assembly to direct centriole architecture.Nature communications · 2021Article
- Human centrosome organization and function in interphase and mitosis.Seminars in cell & developmental biology · 2021Review
- SAPs as a new model to probe the pathway of centriole and centrosome assembly.Biochemical Society transactions · 2021Review
- The dimeric Golgi protein Gorab binds to Sas6 as a monomer to mediate centriole duplication.eLife · 2021Article
- Identification of compounds that bind the centriolar protein SAS-6 and inhibit its oligomerization.The Journal of biological chemistry · 2020Article
- Interaction interface in the C-terminal parts of centriole proteins Sas6 and Ana2.Open biology · 2020Article
- Article
- With Age Comes Maturity: Biochemical and Structural Transformation of a Human Centriole in the Making.Cells · 2020Review
- CCDC61/VFL3 Is a Paralog of SAS6 and Promotes Ciliary Functions.Structure (London, England : 1993) · 2020Article
- Article
- Mechanism and Regulation of Centriole and Cilium Biogenesis.Annual review of biochemistry · 2019Review
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Authors and funding
6 authors.
Funding
Abstract
Sas-6 and Ana2/STIL proteins are required for centriole duplication and the homo-oligomerisation properties of Sas-6 help establish the ninefold symmetry of the central cartwheel that initiates centriole assembly. Ana2/STIL proteins are poorly conserved, but they all contain a predicted Central Coiled-Coil Domain (CCCD). Here we show that the Drosophila Ana2 CCCD forms a tetramer, and we solve its structure to 0.8 Å, revealing that it adopts an unusual parallel-coil topology. We also solve the structure of the Drosophila Sas-6 N-terminal domain to 2.9 Å revealing that it forms higher-order oligomers through canonical interactions. Point mutations that perturb Sas-6 or Ana2 homo-oligomerisation in vitro strongly perturb centriole assembly in vivo. Thus, efficient centriole duplication in flies requires the homo-oligomerisation of both Sas-6 and Ana2, and the Ana2 CCCD tetramer structure provides important information on how these proteins might cooperate to form a cartwheel structure.
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