ArticleeLife2015
Acidic pH and divalent cation sensing by PhoQ are dispensable for systemic salmonellae virulence.
Article in eLife, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 33 citations in OpenAlex.
- Multifaceted Evolution of the PhoPQ Two-Component System in Salmonella enterica Enhanced the Expression of Horizontally Acquired Virulence Genes.Molecular microbiology · 2025Article
- Origins of symbiosis: shared mechanisms underlying microbial pathogenesis, commensalism and mutualism of plants and animals.FEMS microbiology reviews · 2023Review
- An E. coli display method for characterization of peptide-sensor kinase interactions.Nature chemical biology · 2023Article
- The rpoS gene confers resistance to low osmolarity conditions in Salmonella enterica serovar Typhi.PloS one · 2022Article
- Allosteric mechanism of signal transduction in the two-component system histidine kinase PhoQ.eLife · 2021Article
- How the PhoP/PhoQ System Controls Virulence and MgMicrobiology and molecular biology reviews : MMBR · 2021Review
- MgrB Inactivation Confers Trimethoprim Resistance inFrontiers in microbiology · 2021Article
- Regulating polymyxin resistance in Gram-negative bacteria: roles of two-component systems PhoPQ and PmrAB.Future microbiology · 2020Review
- A Novel PhoP/PhoQ Regulation Pathway Modulates the Survival of Extraintestinal PathogenicFrontiers in immunology · 2018Article
- Osmosensing by the bacterial PhoQ/PhoP two-component system.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Activation of master virulence regulator PhoP in acidic pH requires theScience signaling · 2017Article
- Review
- Acidic pH sensing in the bacterial cytoplasm is required for Salmonella virulence.Molecular microbiology · 2016Article
- Discrimination and Integration of Stress Signals by Pathogenic Bacteria.Cell host & microbe · 2016Review
- Bacterial Stress Responses during Host Infection.Cell host & microbe · 2016Review
- Deciphering tissue-induced Klebsiella pneumoniae lipid A structure.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 2 countries.
Funding
Abstract
Salmonella PhoQ is a histidine kinase with a periplasmic sensor domain (PD) that promotes virulence by detecting the macrophage phagosome. PhoQ activity is repressed by divalent cations and induced in environments of acidic pH, limited divalent cations, and cationic antimicrobial peptides (CAMP). Previously, it was unclear which signals are sensed by salmonellae to promote PhoQ-mediated virulence. We defined conformational changes produced in the PhoQ PD on exposure to acidic pH that indicate structural flexibility is induced in α-helices 4 and 5, suggesting this region contributes to pH sensing. Therefore, we engineered a disulfide bond between W104C and A128C in the PhoQ PD that restrains conformational flexibility in α-helices 4 and 5. PhoQ(W104C-A128C) is responsive to CAMP, but is inhibited for activation by acidic pH and divalent cation limitation. phoQ(W104C-A128C) Salmonella enterica Typhimurium is virulent in mice, indicating that acidic pH and divalent cation sensing by PhoQ are dispensable for virulence.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.