Evidence map›Paper›PMID 26001519›Full record

ArticleExtremophiles : life under extreme conditions2015

Calcium alginate matrix increases the stability and recycling capability of immobilized endo-β-1,4-xylanase from Geobacillus stearothermophilus KIBGE-IB29.

Zainab Bibi, Shah Ali Ul Qader, Afsheen Aman

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Article in Extremophiles : life under extreme conditions, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

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8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

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3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Article
  2. Aspergillus labruscus ITAL 22.223 xylanase - immobilization and application for the obtainment of corncob xylan targeting xylitol production.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024
    Article
  3. Article
  4. Article
  5. Prebiotic effect of sorghum biomass xylooligosaccharides employing immobilized endoxylanase from Thermomyces lanuginosus PC7S1T.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2022
    Article
  6. Article
  7. Article
  8. Article
4 · The record

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5 · Who and what money

Authors and funding

3 authors.

Zainab BibiThe Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi, 75270, Pakistan.
Shah Ali Ul Qader
Afsheen Aman

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Exploration of microbial pool from extremely diversified ecosystem is significantly important for various industrial applications. Bacterial communities from extreme habitats including volcanic vents, hot springs, and industrial sectors are eagerly explored for the isolation of thermophiles. Geobacillus stearothermophilus KIBGE-IB29, isolated from blast furnace site of a steel processing industry, is capable of producing thermostable endo-β-1,4-xylanase. In the current study, this enzyme was immobilized within calcium alginate beads using entrapment technique. Amalgamation of sodium alginate (40.0 gL(-1)) and calcium chloride (0.4 M) was used for the formation of immobilized beads. It was observed that temperature (50 °C) and pH (7.0) optima of immobilized enzyme remained same, but enzyme-substrate reaction time increased from 5.0 to 30.0 min as compared to free enzyme. Diffusion limit of high molecular weight xylan (corncob) caused a decline in V max of immobilized enzyme from 4773 to 203.7 U min(-1), whereas K m value increased from 0.5074 to 0.5722 mg ml(-1) with reference to free enzyme. Immobilized endo-β-1,4-xylanase showed its stability even at high temperatures as compared to free enzyme and retained 18 and 9 % residual activity at 70 and 80 °C, respectively. Immobilized enzyme also exhibited sufficient recycling efficiency up to five reaction cycles which indicated that this enzyme can be a plausible candidate in paper and pulp industry.

Indexed as

AlginatesBacterial ProteinsEndo-1,4-beta XylanasesEnzymes, ImmobilizedEnzyme StabilityGeobacillus stearothermophilusGlucuronic AcidHexuronic AcidsAlginatesBacterial ProteinsEndo-1,4-beta XylanasesEnzymes, ImmobilizedGlucuronic AcidHexuronic Acids

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.