ArticlePLoS computational biology2015
Metabolic Needs and Capabilities of Toxoplasma gondii through Combined Computational and Experimental Analysis.
Article in PLoS computational biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed, 106 citations in OpenAlex.
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- In silico analysis and comparison of the metabolic capabilities of different organisms by reducing metabolic complexity.Microbiome · 2026Article
- Immunometabolism at the crossroads of infection: mechanistic and systems-level perspectives from host and pathogen.Immunometabolism (Cobham, Surrey) · 2025Review
- Classically activated macrophages undergo functionally significant nucleotide metabolism remodelling driven by nitric oxide.Nature metabolism · 2025Article
- Macrophages say NO to nucleotide synthesis and salvage instead.Nature metabolism · 2025Article
- Metabolic network reconstruction as a resource for analyzing Salmonella Typhimurium SL1344 growth in the mouse intestine.PLoS computational biology · 2025Article
- An endoplasmic reticulum localized acetyl-CoA transporter is required for efficient fatty acid synthesis inOpen biology · 2024Article
- Metabolic changes inmBio · 2024Article
- The mitochondrion ofProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Development of a new quantification method of Sarcocystis cruzi through detection of the acetyl-CoA synthetase gene.The Journal of veterinary medical science · 2023Article
- Toxoplasma metabolic flexibility in different growth conditions.Trends in parasitology · 2022Review
- Rapid-SL identifies synthetic lethal sets with an arbitrary cardinality.Scientific reports · 2022Article
- Ceramide biosynthesis is critical for establishment of the intracellular niche of Toxoplasma gondii.Cell reports · 2022Article
- merlin, an improved framework for the reconstruction of high-quality genome-scale metabolic models.Nucleic acids research · 2022Article
- Preclinical characterization and target validation of the antimalarial pantothenamide MMV693183.Nature communications · 2022Article
- The flexibility of Apicomplexa parasites in lipid metabolism.PLoS pathogens · 2022Review
- Essential role of pyrophosphate homeostasis mediated by the pyrophosphate-dependent phosphofructokinase in Toxoplasma gondii.PLoS pathogens · 2022Article
- Comparative analyses of parasites with a comprehensive database of genome-scale metabolic models.PLoS computational biology · 2022Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Toxoplasma gondii is a human pathogen prevalent worldwide that poses a challenging and unmet need for novel treatment of toxoplasmosis. Using a semi-automated reconstruction algorithm, we reconstructed a genome-scale metabolic model, ToxoNet1. The reconstruction process and flux-balance analysis of the model offer a systematic overview of the metabolic capabilities of this parasite. Using ToxoNet1 we have identified significant gaps in the current knowledge of Toxoplasma metabolic pathways and have clarified its minimal nutritional requirements for replication. By probing the model via metabolic tasks, we have further defined sets of alternative precursors necessary for parasite growth. Within a human host cell environment, ToxoNet1 predicts a minimal set of 53 enzyme-coding genes and 76 reactions to be essential for parasite replication. Double-gene-essentiality analysis identified 20 pairs of genes for which simultaneous deletion is deleterious. To validate several predictions of ToxoNet1 we have performed experimental analyses of cytosolic acetyl-CoA biosynthesis. ATP-citrate lyase and acetyl-CoA synthase were localised and their corresponding genes disrupted, establishing that each of these enzymes is dispensable for the growth of T. gondii, however together they make a synthetic lethal pair.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.