Evidence map›Paper›PMID 26001072›Full record

ArticleBiomaterials2015

Orthogonally oriented scaffolds with aligned fibers for engineering intestinal smooth muscle.

Masae Kobayashi, Nan Ye Lei, Qianqian Wang, Benjamin M Wu, James C Y Dunn

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Masae KobayashiDepartment of Bioengineering, Henry Samueli School of Engineering, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Nan Ye LeiDepartment of Bioengineering, Henry Samueli School of Engineering, University of California, Los Angeles, Los Angeles, CA 90095, USA; Department of Surgery, David Geffen School of Medicine at UCLA, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Qianqian WangDepartment of Bioengineering, Henry Samueli School of Engineering, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Benjamin M WuDepartment of Bioengineering, Henry Samueli School of Engineering, University of California, Los Angeles, Los Angeles, CA 90095, USA; Division of Advanced Prosthodontics & Weintraub Center for Reconstructive Biotechnology, University of California, Los Angeles, Los Angeles, CA, USA.
James C Y DunnDepartment of Bioengineering, Henry Samueli School of Engineering, University of California, Los Angeles, Los Angeles, CA 90095, USA; Department of Surgery, David Geffen School of Medicine at UCLA, University of California, Los Angeles, Los Angeles, CA 90095, USA. Electronic address: jdunn@mednet.ucla.edu.

Funding

Pilot and Feasibility ProgramP30DK041301 · NIDDK · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ROZENGURT, JUAN ENRIQUE · 1990 to 2019
$18.0M
Intestinal Tissue EngineeringR01DK083319 · NIDDK · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI DUNN, JAMES C · 2010 to 2012
$998k
NIDDK NIH HHS P30 DK041301NIDDK NIH HHS R01 DK083119NIDDK NIH HHS R01 DK083319
6 · The paper itself

Abstract

Controlling cellular alignment is critical in engineering intestines with desired structure and function. Although previous studies have examined the directional alignment of cells on the surface (x-y plane) of parallel fibers, quantitative analysis of the cellular alignment inside implanted scaffolds with oriented fibers has not been reported. This study examined the cellular alignment in the x-z and y-z planes of scaffolds made with two layers of orthogonally oriented fibers. The cellular orientation inside implanted scaffolds was evaluated with immunofluorescence. Quantitative analysis of coherency between cell orientation and fiber direction confirmed that cells aligned along the fibers not only on the surface (x-y plane) but also inside the scaffolds (x-z & y-z planes). Our study demonstrated that two layers of orthogonally aligned scaffolds can generate the histological organization of cells similar to that of intestinal circular and longitudinal smooth muscle.

Indexed as

Tissue ScaffoldsAnimalsAnisotropyCell ProliferationCells, CulturedEquipment DesignEquipment Failure AnalysisIntestinesMaterials TestingMiceMice, Inbred C57BLMuscle, SmoothMyocytes, Smooth MuscleNanofibersTissue EngineeringCellular alignmentElectrospinningPolycaprolactoneScaffoldSmooth muscle cellTissue engineering

Identifiers

PMID26001072
PMCPMC4464968

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.