ArticlePLoS neglected tropical diseases2015
Transcriptome Profiling and Genetic Study Reveal Amplified Carboxylesterase Genes Implicated in Temephos Resistance, in the Asian Tiger Mosquito Aedes albopictus.
Article in PLoS neglected tropical diseases, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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42 citing papers in PubMed, 65 citations in OpenAlex.
- NADPH-cytochrome P450 reductase mediates P450-dependent metabolic resistance to deltamethrin in Aedes albopictus.Parasites & vectors · 2026Article
- Insecticide resistance levels, spatial distribution, and kdr mutations in the dengue vector Aedes albopictus of Hong Kong.PLoS neglected tropical diseases · 2025Article
- Expression of cytochrome P450 monooxygenase genes in pyrethroid-resistant Aedes albopictus.Scientific reports · 2025Article
- Transcriptome and Functional Analyses Revealed the Carboxylesterase Genes Involved in Pyrethroid Resistance inInsects · 2025Article
- Characterization of the transcriptional cellular response in midgut tissue of temephos-resistant Aedes aegypti larvae.Parasites & vectors · 2025Article
- Copy number variants underlie major selective sweeps in insecticide resistance genes in Anopheles arabiensis.PLoS biology · 2024Article
- Whole genome sequence analysis of population structure and insecticide resistance markers in Anopheles melas from the Bijagós Archipelago, Guinea-Bissau.Parasites & vectors · 2024Article
- Uridine diphosphate (UDP)-glycosyltransferases (UGTs) are associated with insecticide resistance in the major malaria vectors Anopheles gambiae s.l. and Anopheles funestus.Scientific reports · 2024Article
- Unveiling candidate genes for metabolic resistance to malathion in Aedes albopictus through RNA sequencing-based transcriptome profiling.PLoS neglected tropical diseases · 2024Article
- Copy number variants underlie the major selective sweeps in insecticide resistance genes inbioRxiv : the preprint server for biology · 2024Article
- Aedes aegypti CCEae3A carboxylase expression confers carbamate, organophosphate and limited pyrethroid resistance in a model transgenic mosquito.PLoS neglected tropical diseases · 2024Article
- Article
- Fitness costs associated with a GABA receptor mutation conferring dieldrin resistance in Aedes albopictus.Heredity · 2022Article
- Field Performance Assessment of IrradiatedFrontiers in bioengineering and biotechnology · 2022Article
- Transcriptomic analysis and molecular docking reveal genes involved in the response of Aedes aegypti larvae to an essential oil extracted from Eucalyptus.PLoS neglected tropical diseases · 2021Article
- Widespread multiple insecticide resistance in the major dengue vector Aedes albopictus in Hainan Province, China.Pest management science · 2021Article
- Bioactivities of rose-scented geranium nanoemulsions against the larvae of Anopheles stephensi and their gut bacteria.PloS one · 2021Article
- Gradual reduction of susceptibility and enhanced detoxifying enzyme activities of laboratory-rearedToxicology reports · 2021Article
- Transcriptional variation of sensory-related genes in natural populations of Aedes albopictus.BMC genomics · 2020Article
- Bioassay and molecular monitoring of insecticide resistance status in Aedes albopictus populations from Greece, to support evidence-based vector control.Parasites & vectors · 2020Article
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Authors and funding
8 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe control of Aedes albopictus, a major vector for viral diseases, such as dengue fever and chikungunya, has been largely reliant on the use of the larvicide temephos for many decades. This insecticide remains a primary control tool for several countries and it is a potential reliable reserve, for emergency epidemics or new invasion cases, in regions such as Europe which have banned its use. Resistance to temephos has been detected in some regions, but the mechanism responsible for the trait has not been investigated. PRINCIPAL
findingsTemephos resistance was identified in an Aedes albopictus population isolated from Greece, and subsequently selected in the laboratory for a few generations. Biochemical assays suggested the association of elevated carboxylesterases (CCE), but not target site resistance (altered AChE), with this phenotype. Illumina transcriptomic analysis revealed the up-regulation of three transcripts encoding CCE genes in the temephos resistant strain. CCEae3a and CCEae6a showed the most striking up-regulation (27- and 12-folds respectively, compared to the reference susceptible strain); these genes have been previously shown to be involved in temephos resistance also in Ae. aegypti. Gene amplification was associated with elevated transcription levels of both CCEae6a and CCEae3a genes. Genetic crosses confirmed the genetic link between CCEae6a and CCEae3a amplification and temephos resistance, by demonstrating a strong association between survival to temephos exposure and gene copy numbers in the F2 generation. Other transcripts, encoding cytochrome P450s, UDP-glycosyltransferases (UGTs), cuticle and lipid biosynthesis proteins, were upregulated in resistant mosquitoes, indicating that the co-evolution of multiple mechanisms might contribute to resistance. SIGNIFICANCE: The identification of specific genes associated with insecticide resistance in Ae. albopictus for the first time is an important pre-requirement for insecticide resistance management. The genomic resources that were produced will be useful to the community, to study relevant aspects of Ae. albopictus biology.
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