ArticlePloS one2015
A customized light sheet microscope to measure spatio-temporal protein dynamics in small model organisms.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 29 citations in OpenAlex.
- Development of Selective Plane Illumination Surface Enhanced Raman Scattering Spectral Microscopy for Improved Cellular Imaging.Journal of Raman spectroscopy : JRS · 2025Article
- What's past is prologue: FRAP keeps delivering 50 years later.Biophysical journal · 2023Review
- Combining multiple fluorescence imaging techniques in biology: when one microscope is not enough.Molecular biology of the cell · 2022Article
- Deep learning-based image processing in optical microscopy.Biophysical reviews · 2022Review
- Meta-lens light-sheet fluorescence microscopy forNanophotonics (Berlin, Germany) · 2022Article
- Low-cost optofluidic add-on enables rapid selective plane illumination microscopy of C. elegans with a conventional wide-field microscope.Journal of biomedical optics · 2021Article
- A noninvasive fluorescence imaging-based platform measures 3D anisotropic extracellular diffusion.Nature communications · 2021Article
- Pharyngeal Pumping and Tissue-Specific Transgenic P-Glycoprotein Expression Influence Macrocyclic Lactone Susceptibility inPharmaceuticals (Basel, Switzerland) · 2021Article
- μSPIM Toolset: A software platform for selective plane illumination microscopy.Journal of neuroscience methods · 2021Article
- Combined Selective Plane Illumination Microscopy and FRAP Maps Intranuclear Diffusion of NLS-GFP.Biophysical journal · 2020Article
- Digging deeper: methodologies for high-content phenotyping in Caenorhabditis elegans.Lab animal · 2019Review
- Fluorescence techniques in developmental biology.Journal of biosciences · 2018Review
- Rapid and gentle hydrogel encapsulation of living organisms enables long-term microscopy over multiple hours.Communications biology · 2018Article
- Phase-Retrieved Tomography enables Mesoscopic imaging of Opaque Tumor Spheroids.Scientific reports · 2017Article
- P-body and Stress Granule Quantification inBio-protocol · 2017Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe a customizable and cost-effective light sheet microscopy (LSM) platform for rapid three-dimensional imaging of protein dynamics in small model organisms. The system is designed for high acquisition speeds and enables extended time-lapse in vivo experiments when using fluorescently labeled specimens. We demonstrate the capability of the setup to monitor gene expression and protein localization during ageing and upon starvation stress in longitudinal studies in individual or small groups of adult Caenorhabditis elegans nematodes. The system is equipped to readily perform fluorescence recovery after photobleaching (FRAP), which allows monitoring protein recovery and distribution under low photobleaching conditions. Our imaging platform is designed to easily switch between light sheet microscopy and optical projection tomography (OPT) modalities. The setup permits monitoring of spatio-temporal expression and localization of ageing biomarkers of subcellular size and can be conveniently adapted to image a wide range of small model organisms and tissue samples.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.