ArticleLab on a chip2015
Passive microfluidic chamber for long-term imaging of axon guidance in response to soluble gradients.
Article in Lab on a chip, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed, 67 citations in OpenAlex.
- Sub-Neuronal Network Profiling of Extracellular Vesicle Release Using a Compartmentalized Neurofluidic Platform.Advanced biology · 2026Article
- Coro1A and TRIM67 collaborate in netrin-dependent neuronal morphogenesis.The Journal of cell biology · 2025Article
- Roll-to-Roll (R2R) High-Throughput Manufacturing of Foil-Based Microfluidic Chips for Neurite Outgrowth Studies.Micromachines · 2025Article
- TRIM9 Controls Growth Cone Responses to Netrin Through DCC and UNC5C.Journal of neurochemistry · 2025Article
- Article
- Adhesion-clutch between DCC and netrin-1 mediates netrin-1-induced axonal haptotaxis.Frontiers in molecular neuroscience · 2024Article
- Investigation of dynamic solution interactions between NET-1 and UNC-5B by multi-wavelength analytical ultracentrifugation.European biophysics journal : EBJ · 2023Article
- Advances in construction and modeling of functional neural circuits in vitro.Neurochemical research · 2022Article
- Neurons-on-a-Chip:Molecules and cells · 2022Review
- Lab-on-Chip Microsystems forFrontiers in bioengineering and biotechnology · 2022Review
- Unraveling Axon Guidance during Axotomy and Regeneration.International journal of molecular sciences · 2021Review
- Manipulating oligodendrocyte intrinsic regeneration mechanism to promote remyelination.Cellular and molecular life sciences : CMLS · 2021Review
- Understanding, engineering, and modulating the growth of neural networks: An interdisciplinary approach.Biophysics reviews · 2021Review
- Experimental Platform to Study Spiking Pattern Propagation in Modular Networks In Vitro.Brain sciences · 2021Article
- Serotonin functions as a bidirectional guidance molecule regulating growth cone motility.Cellular and molecular life sciences : CMLS · 2021Article
- Neurite guidance and neuro-caging on steps and grooves in 2.5 dimensions.Nanoscale advances · 2020Article
- Dscam1 establishes the columnar units through lineage-dependent repulsion between sister neurons in the fly brain.Nature communications · 2020Article
- Mechanistic advances in axon pathfinding.Current opinion in cell biology · 2020Review
- A pair of E3 ubiquitin ligases compete to regulate filopodial dynamics and axon guidance.The Journal of cell biology · 2020Article
- Assessing drug response in engineered brain microenvironments.Brain research bulletin · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 3 institutions in 1 country.
Funding
Abstract
Understanding how axons are guided to target locations within the brain is of fundamental importance for neuroscience, and is a widely studied area of research. Biologists have an unmet need for reliable and easily accessible methods that generate stable, soluble molecular gradients for the investigation of axon guidance. Here we developed a microfluidic device with contiguous media-filled compartments that uses gravity-driven flow to generate a stable and highly reproducible gradient within a viewing compartment only accessible to axons. This device uses high-resistance microgrooves to both direct the growth of axons into an isolated region and to generate a stable gradient within the fluidically isolated axon viewing compartment for over 22 h. Establishing a stable gradient relies on a simple and quick pipetting procedure with no external pump or tubing. Since the axons extend into the axonal compartment through aligned microgrooves, the analysis of turning is simplified. Further, the multiple microgrooves in parallel alignment serve to increase sample sizes, improving statistical analyses. We used this method to examine growth cone turning in response to the secreted axon guidance cue netrin-1. We report the novel finding that growth cones of embryonic mouse cortical axons exhibited attractive turning in the lower concentrations of netrin-1, but were repulsed when exposed to higher concentrations. We also performed immunocytochemistry in growth cones exposed to a netrin-1 gradient within the axon viewing compartment and show that netrin receptors associated with both attraction and repulsion, DCC and UNC5H, localized to these growth cones. Together, we developed an accessible gradient chamber for higher throughput axon guidance studies and demonstrated its capabilities.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.