ArticleFrontiers in microbiology2015
Examining the virulence of Candida albicans transcription factor mutants using Galleria mellonella and mouse infection models.
Article in Frontiers in microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 54 citations in OpenAlex.
- Devising a Zfu2p targeted antifungal strategy that may preserve commensalism while suppressing Candida albicans virulence during vulvovaginal candidiasis.Scientific reports · 2026Article
- A fungal metabolic regulator underlies infectious synergism during Candida albicans-Staphylococcus aureus intra-abdominal co-infection.Nature communications · 2024Article
- Interactions between antifungals and everolimus againstFrontiers in cellular and infection microbiology · 2023Article
- Many ways, one microorganism: Several approaches to study Malassezia in interactions with model hosts.PLoS pathogens · 2022Review
- Candida albicans commensalism in the oral mucosa is favoured by limited virulence and metabolic adaptation.PLoS pathogens · 2022Article
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- Interaction Between Amorolfine and Voriconazole Against Fusarium species.Mycopathologia · 2021Article
- AJournal of fungi (Basel, Switzerland) · 2021Article
- Function Analysis ofFrontiers in fungal biology · 2021Article
- In Vitro or In Vivo Models, the Next Frontier for Unraveling Interactions betweenJournal of fungi (Basel, Switzerland) · 2020Review
- Transcriptional control of hyphal morphogenesis in Candida albicans.FEMS yeast research · 2020Review
- Exploiting Lactoferricin (17-30) as a Potential Antimicrobial and Antibiofilm Candidate Against Multi-Drug-Resistant EnteroaggregativeFrontiers in microbiology · 2020Article
- Article
- Correlating Genotype and Phenotype in the Asexual YeastG3 (Bethesda, Md.) · 2019Article
- Virulence and antifungal susceptibility of microsatellite genotypes of Candida albicans from superficial and deep locations.Yeast (Chichester, England) · 2019Article
- Efficacy of Antifungal Monotherapies and Combinations against Aspergillus calidoustus.Antimicrobial agents and chemotherapy · 2018Article
- Standardization ofJournal of fungi (Basel, Switzerland) · 2018Review
- Systematic Complex Haploinsufficiency-Based Genetic Analysis ofG3 (Bethesda, Md.) · 2018Article
- Experimental in Vivo Models of Candidiasis.Journal of fungi (Basel, Switzerland) · 2018Review
- Galleria mellonella as a host model to study Candida glabrata virulence and antifungal efficacy.Virulence · 2017Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of the present study was to identify Candida albicans transcription factors (TFs) involved in virulence. Although mice are considered the gold-standard model to study fungal virulence, mini-host infection models have been increasingly used. Here, barcoded TF mutants were first screened in mice by pools of strains and fungal burdens (FBs) quantified in kidneys. Mutants of unannotated genes which generated a kidney FB significantly different from that of wild-type were selected and individually examined in Galleria mellonella. In addition, mutants that could not be detected in mice were also tested in G. mellonella. Only 25% of these mutants displayed matching phenotypes in both hosts, highlighting a significant discrepancy between the two models. To address the basis of this difference (pool or host effects), a set of 19 mutants tested in G. mellonella were also injected individually into mice. Matching FB phenotypes were observed in 50% of the cases, highlighting the bias due to host effects. In contrast, 33.4% concordance was observed between pool and single strain infections in mice, thereby highlighting the bias introduced by the "pool effect." After filtering the results obtained from the two infection models, mutants for MBF1 and ZCF6 were selected. Independent marker-free mutants were subsequently tested in both hosts to validate previous results. The MBF1 mutant showed impaired infection in both models, while the ZCF6 mutant was only significant in mice infections. The two mutants showed no obvious in vitro phenotypes compared with the wild-type, indicating that these genes might be specifically involved in in vivo adapt.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.