Evidence map›Paper›PMID 25999679›Full record

ArticleMolecular vision2015

Protein tyrosine phosphatase 1B regulates the activity of retinal pigment epithelial cells.

Zhao-dong Du, Li-ting Hu, Gui-qiu Zhao, Ying Li, Zhi-zhong Ma

Open access · greenAbstract read
PubMed Full text at PMC
In one paragraph

Article in Molecular vision, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.2field-weighted citation impact, top 45% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 9 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Zhao-dong DuDepartment of Ophthalmology, Affiliated Hospital of Qingdao University, Qingdao, China.
Li-ting HuDepartment of Ophthalmology, Affiliated Hospital of Qingdao University, Qingdao, China.
Gui-qiu ZhaoDepartment of Ophthalmology, Affiliated Hospital of Qingdao University, Qingdao, China.
Ying LiPeking University Eye Center, Peking University Third Hospital, Beijing, China.
Zhi-zhong MaPeking University Eye Center, Peking University Third Hospital, Beijing, China.
Peking University Third Hospital · CNQingdao University · CNAffiliated Hospital of Qingdao University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeTo determine whether protein tyrosine phosphatase 1B (PTP1B) is expressed in rat retinal pigment epithelium (RPE) cells, to evaluate whether inhibition of PTP1B contributes to initiation of RPE cells into an active state, and to investigate the signaling pathways involved in this process.

methodsRat retinas were detached by trans-scleral injection of 1.4% sodium hyaluronate into the subretinal space. Immunocytochemistry evaluated the expression of PTP1B in RPE cells located at normal and detached retinas. From the cultured RPE cells treated with TCS-401, cell proliferation was assessed using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetracolium bromide assay, and the protein expression levels of cyclin A and cyclin D1 were determined. The effect of TCS-401 on cell differentiation was confirmed by immunostaining for α-smooth muscle actin and by western blot. Cell migration activity and PTP1B signaling mechanism were determined. Migration Assay was used to evaluate cell migration activity. PTP1B signaling mechanism was determined by use of PD98059 and LY294002.

resultsPTP1B was expressed in the RPE layer of the normal retina. After retinal detachment, weak immunolabeling of PTP1B was seen in the RPE cells. TCS-401 promoted the proliferation and expression of cyclin A and cyclin D1 in RPE cells. TCS-401 induced RPE cells to differentiate toward better contractility and motility. A migration assay proved that inhibiting PTP1B improved the migratory activity of RPE cells. TCS-401 activated extracellular signal-regulated kinase (Erk) and protein kinase B (Akt) phosphorylation. Pretreatment with PD98059 and LY294002 abolished TCS-401-induced activation of Erk, Akt, cell proliferation, and cell migration.

conclusionsPTP1B may be involved in regulating the active state of RPE cells. The inhibition of PTP1B promoted the proliferation, myofibroblast differentiation, and migration of RPE cells, and MEK/Erk and PI3K/Akt signaling pathways played important roles in the proliferation and migration process.

Indexed as

AnimalsCell DifferentiationCell MovementCell ProliferationCells, CulturedChromonesDisease Models, AnimalEnzyme InhibitorsEpithelial-Mesenchymal TransitionFemaleFlavonoidsMaleMAP Kinase Signaling SystemMorpholinesPhosphatidylinositol 3-KinasesProtein Tyrosine Phosphatase, Non-Receptor Type 12-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-oneChromonesEnzyme InhibitorsFlavonoidsMorpholinesPhosphatidylinositol 3-KinasesProtein Tyrosine Phosphatase, Non-Receptor Type 1Proto-Oncogene Proteins c-aktPtpn1 protein, rat

Identifiers

PMID25999679
PMCPMC4431406
OpenAlexW241730311

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.