ArticleNucleic acids research2015
Characterization of human herpesvirus 6A/B U94 as ATPase, helicase, exonuclease and DNA-binding proteins.
Article in Nucleic acids research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 32 citations in OpenAlex.
- Vertebrate-tropism of a cressdnavirus lineage implicated by poxvirus gene capture.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Impact of Host Telomere Length on HHV-6 Integration.Viruses · 2022Article
- Bracoviruses recruit host integrases for their integration into caterpillar's genome.PLoS genetics · 2021Article
- The U94 Gene of Human Herpesvirus 6: A Narrative Review of Its Role and Potential Functions.Cells · 2020Review
- Role for the shelterin protein TRF2 in human herpesvirus 6A/B chromosomal integration.PLoS pathogens · 2020Article
- Review
- Article
- Analysis of Expression Pattern of snoRNAs in Different Cancer Types with Machine Learning Algorithms.International journal of molecular sciences · 2019Article
- Human Herpesvirus 6A and 6B inhibit in vitro angiogenesis by induction of Human Leukocyte Antigen G.Scientific reports · 2018Article
- Viral Proteins U41 and U70 of Human Herpesvirus 6A Are Dispensable for Telomere Integration.Viruses · 2018Article
- Aging-associated latent herpes viral infection in normal Japanese individuals and patients with Werner syndrome.Intractable & rare diseases research · 2018Article
- Review
- Cell Culture Systems To Study Human Herpesvirus 6A/B Chromosomal Integration.Journal of virology · 2017Article
- Stabilization of Telomere G-Quadruplexes Interferes with Human Herpesvirus 6A Chromosomal Integration.Journal of virology · 2017Article
- Chromosomally Integrated Human Herpesvirus 6: Models of Viral Genome Release from the Telomere and Impacts on Human Health.Viruses · 2017Review
- Telomeres and Telomerase: Role in Marek's Disease Virus Pathogenesis, Integration and Tumorigenesis.Viruses · 2017Review
- Article
- HHV-6A/B Integration and the Pathogenesis Associated with the Reactivation of Chromosomally Integrated HHV-6A/B.Viruses · 2017Review
- Expression of the Human Herpesvirus 6A Latency-Associated Transcript U94A Disrupts Human Oligodendrocyte Progenitor Migration.Scientific reports · 2017Article
- Human Herpesvirus 6B Induces Hypomethylation on Chromosome 17p13.3, Correlating with Increased Gene Expression and Virus Integration.Journal of virology · 2017Article
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
Human herpesvirus-6A (HHV-6A) and HHV-6B integrate their genomes into the telomeres of human chromosomes, however, the mechanisms leading to integration remain unknown. HHV-6A/B encode a protein that has been proposed to be involved in integration termed U94, an ortholog of adeno-associated virus type 2 (AAV-2) Rep68 integrase. In this report, we addressed whether purified recombinant maltose-binding protein (MBP)-U94 fusion proteins of HHV-6A/B possess biological functions compatible with viral integration. We could demonstrate that MBP-U94 efficiently binds both dsDNA and ssDNA containing telomeric repeats using gel shift assay and surface plasmon resonance. MBP-U94 is also able to hydrolyze adenosine triphosphate (ATP) to ADP, providing the energy for further catalytic activities. In addition, U94 displays a 3' to 5' exonuclease activity on dsDNA with a preference for 3'-recessed ends. Once the DNA strand reaches 8-10 nt in length, the enzyme dissociates it from the complementary strand. Lastly, MBP-U94 compromises the integrity of a synthetic telomeric D-loop through exonuclease attack at the 3' end of the invading strand. The preferential DNA binding of MBP-U94 to telomeric sequences, its ability to hydrolyze ATP and its exonuclease/helicase activities suggest that U94 possesses all functions required for HHV-6A/B chromosomal integration.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.