ArticleCytotechnology2016
Establishment and characterization of three embryonic cell lines of beet armyworm, Spodoptera exigua (Lepidoptera: Noctuidae).
Article in Cytotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 14 citations in OpenAlex.
- Article
- The Expression of P35 Plays a Key Role in the Difference in Apoptosis Induced by AcMNPV Infection in DifferentInternational journal of molecular sciences · 2023Article
- Establishing and characterising a new cell line fromHeliyon · 2022Article
- A database of crop pest cell lines.In vitro cellular & developmental biology. Animal · 2022Article
- Establishment of an immortalized cell line derived from the pupal ovary of Mythimna separata (Lepidoptera: Noctuidae) and identification of the cell source.Cell and tissue research · 2021Article
- Establishment and characterization of a new embryonic cell line from Helicoverpa armigera (Hübner).In vitro cellular & developmental biology. Animal · 2020Article
- A cell clone strain from Mythimna separata (Lepidoptera: Noctuidae) highly susceptible to Autographa californica multiple nucleopolyhedrovirus (AcMNPV) and M. separata NPV (MsNPV).In vitro cellular & developmental biology. Animal · 2017Article
- A new cell line derived from embryonic tissues of Holotrichia parallela (Coleoptera:Scarabaeidae).In vitro cellular & developmental biology. Animal · 2016Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Three cell lines (QAU-Se-E-1, -2 and -3, or Se-1, -2 and -3 for short) were established from eggs of beet armyworm (Spodoptera exigua) that have been passaged stably for more than 60 times in TNM-FH medium supplemented with 10 % fetal bovine serum. The cell lines consisted of round and spindle-shaped cells. The round cells accounted for 96.82, 84.34 and 83.16 % of the cells in the three cell lines, respectively, with cell diameters of 16.21 ± 0.72, 15.63 ± 0.58 and 13.06 ± 0.44 μm. Random amplified polymorphic DNA and analysis of the CO I gene showed that the three cell lines were all derived from S. exigua. Growth curves at passage 30 were determined and the results showed that the cell population doubling times were 59.03, 49.08 and 49.91 h, respectively. The three cell lines can be infected by S. exigua multiple nucleopolyhedrovirus (SeMNPV). Se-3 was extremely susceptible to the virus with an infection rate of 97.52 % 4 days after the inoculation and produced 2.02 × 10(6) OBs per mL of culture. Flow cytometry analysis showed that some of Se-1 and Se-2 cells had apoptosis after infection, whereas Se-3 cells did not. Bioassays showed that the virulence of the SeMNPV proliferated from Se-3 was similar to that from the insect with LC50 of 5.55 × 10(5) and 2.64 × 10(5) OBs/mL. Therefore, the cell lines can be used to study the SeMNPV-host interactions and mechanisms underlying the interactions.
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