Evidence map›Paper›PMID 25998827›Full record

ArticleExperimental & molecular medicine2015

MiR-155 modulates the inflammatory phenotype of intestinal myofibroblasts by targeting SOCS1 in ulcerative colitis.

Surajit Pathak, Alessia Rosaria Grillo, Melania Scarpa, Paola Brun, Renata D'Incà, Laura Nai, Antara Banerjee, Donatella Cavallo, Luisa Barzon, Giorgio Palù and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Experimental & molecular medicine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers.

0numbers the graph read from it
0cells of the map it votes in
79citing papers in PubMed
4.7field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

79 citing papers in PubMed, 120 citations in OpenAlex.

  1. Dicer-independent miR-451a is upregulated in periodontitis and potentiates inflammation by impairing macrophage polarization and SOCS activity.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2026
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  8. Milk-Derived Extracellular Vesicles and microRNAs: Potential Modulators of Intestinal Homeostasis.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025
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19 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 2 institutions in 1 country.

Surajit Pathak1] Department of Surgery Oncology and Gastroenterology DISCOG, University of Padova, Padova, Italy [2] Department of Molecular Medicine, University of Padova, Padova, Italy.
Alessia Rosaria Grillo1] Department of Surgery Oncology and Gastroenterology DISCOG, University of Padova, Padova, Italy [2] Department of Molecular Medicine, University of Padova, Padova, Italy.
Melania ScarpaOncological Surgery Unit, Veneto Institute of Oncology IOV-IRCCS, Padova, Italy.
Paola BrunDepartment of Molecular Medicine, University of Padova, Padova, Italy.
Renata D'IncàDepartment of Surgery Oncology and Gastroenterology DISCOG, University of Padova, Padova, Italy.
Laura Nai1] Department of Surgery Oncology and Gastroenterology DISCOG, University of Padova, Padova, Italy [2] Department of Molecular Medicine, University of Padova, Padova, Italy.
Antara BanerjeeDepartment of Surgery Oncology and Gastroenterology DISCOG, University of Padova, Padova, Italy.
Donatella CavalloDepartment of Molecular Medicine, University of Padova, Padova, Italy.
Luisa BarzonDepartment of Molecular Medicine, University of Padova, Padova, Italy.
Giorgio PalùDepartment of Molecular Medicine, University of Padova, Padova, Italy.
Giacomo Carlo SturnioloDepartment of Surgery Oncology and Gastroenterology DISCOG, University of Padova, Padova, Italy.
Andrea BudaDepartment of Surgery Oncology and Gastroenterology DISCOG, University of Padova, Padova, Italy.
Ignazio CastagliuoloDepartment of Molecular Medicine, University of Padova, Padova, Italy.
University of Padua · ITIstituti di Ricovero e Cura a Carattere Scientifico · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Abnormal levels of microRNA (miR)-155, which regulate inflammation and immune responses, have been demonstrated in the colonic mucosa of patients with inflammatory bowel diseases (IBD), although its role in disease pathophysiology is unknown. We investigated the role of miR-155 in the acquisition and maintenance of an activated phenotype by intestinal myofibroblasts (IMF), a key cell population contributing to mucosal damage in IBD. IMF were isolated from colonic biopsies of healthy controls, ulcerative colitis (UC) and Crohn's disease (CD) patients. MiR-155 in IMF was quantified by quantitative reverse transcription-PCR in basal condition and following exposure to TNF-α, interleukin (IL)-1β, lipopolysaccharide (LPS) or TGF-β1. The effects of miR-155 mimic or inhibitor transfection on cytokine release and suppressor of cytokine signaling 1 (SOCS1) expression were assessed by enzyme-linked immunosorbent assay and western blot, respectively. Regulation of the target gene SOCS1 expression by miR-155 was assessed using luciferase reporter construct. We found that miR-155 was significantly upregulated in UC as compared with control- and CD-derived IMF. Moreover, TNF-α and LPS, but not TGF-β1 and IL-1β, significantly increased miR-155 expression in IMF. Ectopic expression of miR-155 in control IMF augmented cytokines release, whereas it downregulated SOCS1 expression. MiR-155 knockdown in UC-IMF reduced cytokine production and enhanced SOCS1 expression. Luciferase reporter assay demonstrated that miR-155 directly targets SOCS1. Moreover, silencing of SOCS1 in control IMF significantly increased IL-6 and IL-8 release. In all, our data suggest that inflammatory mediators induce miR-155 expression in IMF of patients with UC. By downregulating the expression of SOCS1, miR-155 wires IMF inflammatory phenotype.

Indexed as

Gene Expression RegulationAdultAgedCells, CulturedColitis, UlcerativeCytokinesFemaleHumansIntestinal MucosaMaleMicroRNAsMiddle AgedMyofibroblastsSuppressor of Cytokine Signaling 1 ProteinSuppressor of Cytokine Signaling ProteinsTumor Necrosis Factor-alphaCytokinesMicroRNAsMIRN155 microRNA, humanSOCS1 protein, humanSuppressor of Cytokine Signaling 1 ProteinSuppressor of Cytokine Signaling ProteinsTumor Necrosis Factor-alpha

Identifiers

PMID25998827
PMCPMC4454995
OpenAlexW608634786

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-SA
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.