ArticleInfection control and hospital epidemiology2015
Whole-genome sequencing for outbreak investigations of methicillin-resistant Staphylococcus aureus in the neonatal intensive care unit: time for routine practice?
Article in Infection control and hospital epidemiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Molecular epidemiology of methicillin-resistantFrontiers in microbiology · 2025Review
- Virulence attributes of successful methicillin-resistantClinical microbiology reviews · 2023Review
- Strain-resolved metagenomic analysis of the gut as a reservoir for bloodstream infection pathogens among premature infants in Singapore.Gut pathogens · 2023Article
- BacARscan: anBiology methods & protocols · 2022Article
- Use of Pulsed-Field Gel Electrophoresis to Determine the Source of Methicillin-ResistantInfectious disease reports · 2021Article
- Genomic Epidemiology and Global Population Structure of Exfoliative Toxin A-ProducingFrontiers in microbiology · 2021Article
- A Complete Genome Screening Program of Clinical Methicillin-Resistant Staphylococcus aureus Isolates Identifies the Origin and Progression of a Neonatal Intensive Care Unit Outbreak.Journal of clinical microbiology · 2019Article
- Improved characterisation of MRSA transmission using within-host bacterial sequence diversity.eLife · 2019Article
- Status and potential of bacterial genomics for public health practice: a scoping review.Implementation science : IS · 2019Article
- Communicable Disease Surveillance Ethics in the Age of Big Data and New Technology.Asian bioethics review · 2019Article
- Intra-Hospital, Inter-Hospital and Intercontinental Spread of ST78 MRSA From Two Neonatal Intensive Care Unit Outbreaks Established Using Whole-Genome Sequencing.Frontiers in microbiology · 2018Article
- Routine Whole-Genome Sequencing for Outbreak Investigations ofFrontiers in microbiology · 2018Article
- Bacterial genome sequencing in clinical microbiology: a pathogen-oriented review.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2017Review
- Infant Colonization With Methicillin-Resistant Staphylococcus aureus or Vancomycin-Resistant Enterococci Preceding Neonatal Intensive Care Unit Discharge.Journal of the Pediatric Infectious Diseases Society · 2017Article
- Outbreaks in the neonatal ICU: a review of the literature.Current opinion in infectious diseases · 2017Review
- Multi-drug resistant Klebsiella pneumoniae strains circulating in hospital setting: whole-genome sequencing and Bayesian phylogenetic analysis for outbreak investigations.Scientific reports · 2017Article
- Phylogenetic Insight into Zika and Emerging Viruses for a Perspective on Potential Hosts.EcoHealth · 2017Article
- Comparison of Whole-Genome Sequencing Methods for Analysis of Three Methicillin-Resistant Staphylococcus aureus Outbreaks.Journal of clinical microbiology · 2017Article
- Article
- New Developments in Clinical Bacteriology Laboratories.Mayo Clinic proceedings · 2016Review
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
BACKGROUND Infants in the neonatal intensive care unit (NICU) are at increased risk for methicillin-resistant Staphylococcus aureus (MRSA) acquisition. Outbreaks may be difficult to identify due in part to limitations in current molecular genotyping available in clinical practice. Comparison of genome-wide single nucleotide polymorphisms (SNPs) may identify epidemiologically distinct isolates among a population sample that appears homogenous when evaluated using conventional typing methods. OBJECTIVE To investigate a putative MRSA outbreak in a NICU utilizing whole-genome sequencing and phylogenetic analysis to identify recent transmission events. DESIGN Clinical and surveillance specimens collected during clinical care and outbreak investigation. PATIENTS A total of 17 neonates hospitalized in a 43-bed level III NICU in northeastern Florida from December 2010 to October 2011 were included in this study. METHODS We assessed epidemiological data in conjunction with 4 typing methods: antibiograms, PFGE, spa types, and phylogenetic analysis of genome-wide SNPs. RESULTS Among the 17 type USA300 isolates, 4 different spa types were identified using pulsed-field gel electrophoresis. Phylogenetic analysis identified 5 infants as belonging to 2 clusters of epidemiologically linked cases and excluded 10 unlinked cases from putative transmission events. The availability of these results during the initial investigation would have improved infection control interventions. CONCLUSION Whole-genome sequencing and phylogenetic analysis are invaluable tools for epidemic investigation; they identify transmission events and exclude cases mistakenly implicated by traditional typing methods. When routinely applied to surveillance and investigation in the clinical setting, this approach may provide actionable intelligence for measured, appropriate, and effective interventions.
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