ArticlePloS one2015
Hydrogen and oxygen stable isotope fractionation in body fluid compartments of dairy cattle according to season, farm, breed, and reproductive stage.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed, 27 citations in OpenAlex.
- Multi-Country Study of Stable Isotopes and Mineral Elements in European Pork.Foods (Basel, Switzerland) · 2026Article
- Local circulation of elites punctuated by transregional mobility enabled steppe political consolidation in the Xiongnu nomadic state.PloS one · 2024Article
- Verifying origin claims on dairy products using stable isotope ratio analysis and random forest classification.Food chemistry: X · 2023Article
- Isotope partitioning between cow milk and farm water: A tool for verification of milk provenance.Rapid communications in mass spectrometry : RCM · 2021Article
- A pilot study of eDNA metabarcoding to estimate plant biodiversity by an alpine glacier core (Adamello glacier, North Italy).Scientific reports · 2021Article
- Milk Authentication: Stable Isotope Composition of Hydrogen and Oxygen in Milks and Their Constituents.Molecules (Basel, Switzerland) · 2020Article
- Feasibility of Casein to Record Stable Isotopic Variation of Cow Milk in New Zealand.Molecules (Basel, Switzerland) · 2020Article
- Growing up in Ancient Sardinia: Infant-toddler dietary changes revealed by the novel use of hydrogen isotopes (δ2H).PloS one · 2020Article
- Possible Mechanisms of Biological Effects Observed in Living Systems duringMolecules (Basel, Switzerland) · 2019Review
- The stable oxygen isotope ratio of resin extractable phosphate derived from fresh cattle faeces.Rapid communications in mass spectrometry : RCM · 2018Article
- Model explanation of the seasonal variation of δScientific reports · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Environmental temperature affects water turnover and isotope fractionation by causing water evaporation from the body in mammals. This may lead to rearrangement of the water stable isotope equilibrium in body fluids. We propose an approach to detect possible variations in the isotope ratio in different body fluids on the basis of different homoeothermic adaptations in varying reproductive stages. Three different reproductive stages (pregnant heifer, primiparous lactating cow, and pluriparous lactating cow) of two dairy cattle breeds (Italian Friesian and Modenese) were studied in winter and summer. Blood plasma, urine, faecal water, and milk were sampled and the isotope ratios of H (2H/1H) and O (18O/16O) were determined. Deuterium excess and isotope-fractionation factors were calculated for each passage from plasma to faeces, urine and milk. The effects of the season, reproductive stages and breed on δ2H and δ18O were significant in all the fluids, with few exceptions. Deuterium excess was affected by season in all the analysed fluids. The correlations between water isotope measurements in bovine body fluids ranged between 0.6936 (urine-milk) and 0.7848 (urine-plasma) for δ2H, and between 0.8705 (urine-milk) and 0.9602 (plasma-milk) for δ18O. The increase in both isotopic δ values in all body fluids during summer is representative of a condition in which fractionation took place as a consequence of a different ratio between ingested and excreted water, which leads to an increased presence of the heavy isotopes. The different body water turnover between adult lactating cattle and non-lactating heifers was confirmed by the higher isotopic δ for the latter, with a shift in the isotopic equilibrium towards values more distant from those of drinking water.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.