Evidence map›Paper›PMID 25996545›Full record

ArticleScientific reports2015

Roles of small RNAs in the effects of nutrition on apoptosis and spermatogenesis in the adult testis.

Yongjuan Guan, Guanxiang Liang, Penelope A R Hawken, Irek A Malecki, Greg Cozens, Philip E Vercoe, Graeme B Martin, Le Luo Guan

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
1.8field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 48 citations in OpenAlex.

  1. Review
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  5. Paternal effects on fetal programming.Animal reproduction · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 3 countries.

Yongjuan GuanUWA Institute of Agriculture and School of Animal Biology, University of Western Australia, 35 Stirling Highway, Crawley, WA 6009.
Guanxiang LiangDepartment of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, T6G 2P5, Canada.
Penelope A R HawkenUWA Institute of Agriculture and School of Animal Biology, University of Western Australia, 35 Stirling Highway, Crawley, WA 6009.
Irek A Malecki1] UWA Institute of Agriculture and School of Animal Biology, University of Western Australia, 35 Stirling Highway, Crawley, WA 6009 [2] Department of Animal Sciences, University of Stellenbosch, Matieland 7600, South Africa.
Greg CozensSchool of Anatomy, Physiology and Human Biology, University of Western Australia, 35 Stirling Highway, Crawley, WA 6009.
Philip E VercoeUWA Institute of Agriculture and School of Animal Biology, University of Western Australia, 35 Stirling Highway, Crawley, WA 6009.
Graeme B Martin1] UWA Institute of Agriculture and School of Animal Biology, University of Western Australia, 35 Stirling Highway, Crawley, WA 6009 [2] Nuffield Department of Obstetrics &Gynaecology, University of Oxford, Oxford OX3 9DU, UK.
Le Luo GuanDepartment of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, T6G 2P5, Canada.
University of Western Australia · AUUniversity of Alberta · CAUniversity of Oxford · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We tested whether reductions in spermatozoal quality induced by under-nutrition are associated with increased germ cell apoptosis and disrupted spermatogenesis, and whether these effects are mediated by small RNAs. Groups of 8 male sheep were fed for a 10% increase or 10% decrease in body mass over 65 days. Underfeeding increased the number of apoptotic germ cells (P < 0.05) and increased the expression of apoptosis-related genes (P < 0.05) in testicular tissue. We identified 44 miRNAs and 35 putative piRNAs that were differentially expressed in well-fed and underfed males (FDR < 0.05). Some were related to reproductive system development, apoptosis (miRNAs), and sperm production and quality (piRNAs). Novel-miR-144 (miR-98), was found to target three apoptotic genes (TP53, CASP3, FASL). The proportion of miRNAs as a total of small RNAs was greater in well-fed males than in underfed males (P < 0.05) and was correlated (r = 0.8, P < 0.05) with the proportion of piRNAs in well-fed and underfed males. In conclusion, the reductions in spermatozoal quality induced by under-nutrition are caused, at least partly, by disruptions to Sertoli cell function and increased germ cell apoptosis, mediated by changes in the expression of miRNAs and piRNAs.

Indexed as

ApoptosisSpermatogenesisAnimalsDietMaleMicroRNAsNutritional StatusReal-Time Polymerase Chain ReactionRNA, Small InterferingSertoli CellsSheepSpermatozoaTestisMicroRNAsRNA, Small Interfering

Identifiers

PMID25996545
PMCPMC4440528
OpenAlexW2255054576

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.