ArticleHistochemistry and cell biology2015
Prolonged overexpression of Wnt10b induces epidermal keratinocyte transformation through activating EGF pathway.
Article in Histochemistry and cell biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 21 citations in OpenAlex.
- Wnt10b Regulation of Hair Follicle Development, Regeneration, and Skin Diseases.Stem cell reviews and reports · 2025Review
- Progress on mitochondria and hair follicle development in androgenetic alopecia: relationships and therapeutic perspectives.Stem cell research & therapy · 2025Review
- [Research progress in regulation of hair growth by dermal adipose tissue].Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2024Review
- The role of WNT10B in physiology and disease: A 10-year update.Frontiers in cell and developmental biology · 2023Review
- Regulation of signaling pathways in hair follicle stem cells.Burns & trauma · 2022Review
- WNT7A Promotes EGF-Induced Migration of Oral Squamous Cell Carcinoma Cells by Activating β-Catenin/MMP9-Mediated Signaling.Frontiers in pharmacology · 2020Article
- Suppression of IGF1R in Melanoma Cells by an Adenovirus-Mediated One-Step Knockdown System.Molecular therapy. Nucleic acids · 2018Article
- Gsdma3 regulates hair follicle differentiation via Wnt5a-mediated non-canonical Wnt signaling pathway.Oncotarget · 2017Article
- Article
- Hair follicle stem cell proliferation, Akt and Wnt signaling activation in TPA-induced hair regeneration.Histochemistry and cell biology · 2017Article
- Paracrine Secreted Frizzled-Related Protein 4 Inhibits Melanocytes Differentiation in Hair Follicle.Stem cells international · 2017Article
- The Histochemistry and Cell Biology omnium-gatherum: the year 2015 in review.Histochemistry and cell biology · 2016Review
- Wnt5a Suppresses β-catenin Signaling during Hair Follicle Regeneration.International journal of medical sciences · 2016Article
- Immunohistochemical study of hair follicle stem cells in regenerated hair follicles induced by Wnt10b.International journal of medical sciences · 2016Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
Abstract
Wnt10b is a signaling protein regulating skin development and homeostasis, and the expression of Wnt10b is restricted to epidermal keratinocytes in embryonic and postnatal skin. Recent studies indicate an elevated expression of Wnt10b in skin tumors. However, how Wnt10b regulates skin tumorigenesis remains largely unknown. Here we report that continuous expression of Wnt10b mediates transformation of epidermal keratinocytes through activating genes involved in EGF/MAPK signaling pathways. We first established a prolonged Wnt10b overexpression system in JB6P- cells to represent the elevated Wnt10b expression level in skin keratinocytes. Through expression assays and observations under phase-contrast microscopy, prolonged expression of Wnt10b activated Wnt/β-catenin pathway and induced morphological changes of cells showing longer protrusions and multilayer growth, indicating early-stage cell transformation. Wnt10b also increased cellular proliferation and migration according to BrdU incorporation and cell mobility assays. Furthermore, multi-doses of AdWnt10b treatment to JB6P- cells induced colony formation, stronger invasive ability in transwell system, and anchorage-independent growth in agar gel. In molecular level, AdWnt10b treatment induced increased transcriptional expressions of Egf, downstream Mapk pathway factors, and MMPs. Administration of Wnt antagonist DKK1 blocked the tumor promotion process induced by Wnt10b. Taken together, these findings clearly demonstrate that Wnt10b promotes epidermal keratinocyte transformation through induced Egf pathway.
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