ArticleGenome biology and evolution2015
Dual Organellar Targeting of Aminoacyl-tRNA Synthetases in Diatoms and Cryptophytes.
Article in Genome biology and evolution, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 48 citations in OpenAlex.
- Complete chloroplast genome of Ecklonia maxima and comparative analysis with related species.BMC genomics · 2025Article
- How to upgrade stolen organelles into permanent plastids: A comparative transcriptomic perspective.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- ASAFind 2.0: multi-class protein targeting prediction for diatoms and algae with complex plastids.The Plant journal : for cell and molecular biology · 2025Article
- Localization of heme biosynthesis in the diatomFrontiers in plant science · 2025Article
- Article
- AARS Online: A collaborative database on the structure, function, and evolution of the aminoacyl-tRNA synthetases.IUBMB life · 2024Article
- Characterization of a novel heterozygous variant in the histidyl-tRNA synthetase gene associated with Charcot-Marie-Tooth disease type 2W.IUBMB life · 2024Article
- A cryptic plastid and a novel mitochondrial plasmid inOpen biology · 2024Article
- Towards a Cure for HARS Disease.Genes · 2023Review
- Complete Plastome ofGenes · 2022Article
- An Enigmatic Stramenopile Sheds Light on Early Evolution in Ochrophyta Plastid Organellogenesis.Molecular biology and evolution · 2022Article
- Evolutionary Adjustment of tRNA Identity Rules in Bacillariophyta for Recognition by an Aminoacyl-tRNA Synthetase Adds a Facet to the Origin of Diatoms.Journal of molecular evolution · 2022Article
- Inferred Subcellular Localization of Peroxisomal Matrix Proteins ofFrontiers in plant science · 2022Article
- A metabolic, phylogenomic and environmental atlas of diatom plastid transporters from the model speciesFrontiers in plant science · 2022Article
- The genome of a nonphotosynthetic diatom provides insights into the metabolic shift to heterotrophy and constraints on the loss of photosynthesis.The New phytologist · 2021Article
- Article
- PhaeoNet: A Holistic RNAseq-Based Portrait of Transcriptional Coordination in the Model DiatomFrontiers in plant science · 2020Article
- Article
- Metabolic Innovations Underpinning the Origin and Diversification of the Diatom Chloroplast.Biomolecules · 2019Review
- Organellar DNA Polymerases in Complex Plastid-Bearing Algae.Biomolecules · 2019Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
Abstract
The internal compartmentation of eukaryotic cells not only allows separation of biochemical processes but it also creates the requirement for systems that can selectively transport proteins across the membrane boundaries. Although most proteins function in a single subcellular compartment, many are able to enter two or more compartments, a phenomenon known as dual or multiple targeting. The aminoacyl-tRNA synthetases (aaRSs), which catalyze the ligation of tRNAs to their cognate amino acids, are particularly prone to functioning in multiple subcellular compartments. They are essential for translation, so they are required in every compartment where translation takes place. In diatoms, there are three such compartments, the plastid, the mitochondrion, and the cytosol. In cryptophytes, translation also takes place in the periplastid compartment (PPC), which is the reduced cytoplasm of the plastid's red algal ancestor and which retains a reduced red algal nucleus. We searched the organelle and nuclear genomes of the cryptophyte Guillardia theta and the diatoms Phaeodactylum tricornutum and Thalassiosira pseudonana for aaRS genes and found an insufficient number of genes to provide each compartment with a complete set of aaRSs. We therefore inferred, with support from localization predictions, that many aaRSs are dual targeted. We tested four of the predicted dual targeted aaRSs with green fluorescent protein fusion localizations in P. tricornutum and found evidence for dual targeting to the mitochondrion and plastid in P. tricornutum and G. theta, and indications for dual targeting to the PPC and cytosol in G. theta. This is the first report of dual targeting in diatoms or cryptophytes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.