ArticleBMC microbiology2015
New insights in Staphylococcus pseudintermedius pathogenicity: antibiotic-resistant biofilm formation by a human wound-associated strain.
Article in BMC microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 61 citations in OpenAlex.
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- A novel case of Staphylococcus pseudintermedius aortitis.Access microbiology · 2025Article
- Investigation on biofilm composition and virulence traits of S. pseudintermedius isolated from infected and colonized dogs.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
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- Antimicrobial activity of α-mangostin againstFrontiers in cellular and infection microbiology · 2023Article
- Multi-epitope chimeric vaccine designing and novel drug targets prioritization against multi-drug resistantFrontiers in microbiology · 2022Article
- Antibiotic resistance, biofilm formation, and virulence factors of isolates ofFrontiers in veterinary science · 2022Article
- Formulation and Evaluation of Topical Biodegradable Films Loaded with Levofloxacin Lipid Nanocarriers.AAPS PharmSciTech · 2021Article
- Anisotropic Silver Nanoparticles Gel Exhibits Antibacterial Action and Reduced Scar Formation on Wounds Contaminated with Methicillin-ResistantAnimals : an open access journal from MDPI · 2021Article
- Antibiotic Resistance Profile and Biofilm Production ofPharmaceuticals (Basel, Switzerland) · 2021Article
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- Synergistic Effect of Abietic Acid with Oxacillin against Methicillin-ResistantAntibiotics (Basel, Switzerland) · 2021Article
- Virulence factors in coagulase-positive staphylococci of veterinary interest other thanThe veterinary quarterly · 2020Review
- Occurrence and Characteristics of Methicillin-Resistant and -SusceptibleFood science of animal resources · 2020Article
- Staphylococcus pseudintermedius: an undocumented, emerging pathogen in humans.GMS hygiene and infection control · 2020Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundStaphylococcus pseudintermedius is an opportunistic pathogen recognized as the leading cause of skin, ear, and post-operative bacterial infections in dogs and cats. Zoonotic infections have also recently been reported causing endocarditis, infection of surgical wounds, rhinosinusitis, and catheter-related bacteremia. The aim of the present study is to evaluate, for the first time, the pathogenic potential of S. pseudintermedius isolated from a human infection. To this end, strain DSM 25713, which was recently isolated from a wound of a leukemic patient who underwent a bone marrow transplantation, was investigated for biofilm formation and antibiotic-resistance under conditions relevant for wound infection.
resultsThe effect of pH (5.5, 7.1, and 8.7) and the presence of serum (diluted at 1:2, 1:10, and 1:100) on biofilm formation was assessed through a crystal violet assay. The presence of serum significantly reduced the ability to form biofilm, regardless of the pH value tested. In vitro activity of eight antibiotics against biofilm formation and mature 48 h-old biofilms was comparatively assessed by crystal violet assay and viable cell count, respectively. Antibiotics at sub-inhibitory concentrations reduced biofilm formation in a dose-dependent manner, although cefoxitin was the most active, causing a significant reduction already at 1/8xMIC. Rifampicin showed the highest activity against preformed biofilms (MBEC90: 2xMIC). None of the antibiotics completely eradicated the preformed biofilms, regardless of tested concentrations. Confocal and electron microscopy analyses of mature biofilm revealed a complex "mushroom-like" architecture consisting of microcolonies embedded in a fibrillar extracellular matrix.
conclusionsFor the first time, our results show that human wound-associated S. pseudintermedius is able to form inherently antibiotic-resistant biofilms, suggestive of its pathogenic potential, and consistent with recent reports of zoonotic infections.
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