ArticleScientific reports2015
Cuticular protein with a low complexity sequence becomes cross-linked during insect cuticle sclerotization and is required for the adult molt.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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34 citing papers in PubMed, 104 citations in OpenAlex.
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- Exploring the structure and dynamics of soft and hard cuticle of Bombyx mori using solid-state NMR techniques.Scientific reports · 2024Article
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- Screening of morphology-related genes based on predator-induced transcriptome sequencing and the functional analysis ofCurrent zoology · 2024Article
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- Global Gene Expression Analysis Reveals Complex Cuticle Organization of the Tribolium Compound Eye.Genome biology and evolution · 2023Article
- Effect of Tachinid ParasitoidFrontiers in physiology · 2022Article
- Transcriptome Analysis Reveals the Molecular Response to Salinity Challenge in Larvae of the Giant Freshwater PrawnFrontiers in physiology · 2022Article
- Full spectrum fluorescence lifetime imaging with 0.5 nm spectral and 50 ps temporal resolution.Nature communications · 2021Article
- Physiological characterization of chitin synthase A responsible for the biosynthesis of cuticle chitin in Culex pipiens pallens (Diptera: Culicidae).Parasites & vectors · 2021Article
- Lepidopteran wing scales contain abundant cross-linked film-forming histidine-rich cuticular proteins.Communications biology · 2021Article
- RNAi-Mediated Knockdown of Imaginal Disc Growth Factors (IDGFs) Genes Causes Developmental Malformation and Mortality in Melon Fly,Frontiers in genetics · 2021Article
- Yellow-y Functions in Egg Melanization and Chorion Morphology of the Asian Tiger Mosquito,Frontiers in cell and developmental biology · 2021Article
- Tweedle proteins form extracellular two-dimensional structures defining body and cell shape inOpen biology · 2020Article
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7 authors at 2 institutions in 2 countries.
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No grant is acknowledged in the PubMed record.
Abstract
In the insect cuticle, structural proteins (CPs) and the polysaccharide chitin are the major components. It has been hypothesized that CPs are cross-linked to other CPs and possibly to chitin by quinones or quinone methides produced by the laccase2-mediated oxidation of N-acylcatechols. In this study we investigated functions of TcCP30, the third most abundant CP in protein extracts of elytra (wing covers) from Tribolium castaneum adults. The mature TcCP30 protein has a low complexity and highly polar amino acid sequence. TcCP30 is localized with chitin in horizontal laminae and vertically oriented columnar structures in rigid cuticles, but not in soft and membranous cuticles. Immunoblot analysis revealed that TcCP30 undergoes laccase2-mediated cross-linking during cuticle maturation in vivo, a process confirmed in vitro using recombinant rTcCP30. We identified TcCPR27 and TcCPR18, the two most abundant proteins in the elytra, as putative cross-linking partners of TcCP30. RNAi for the TcCP30 gene had no effect on larval and pupal growth and development. However, during adult eclosion, ~70% of the adults were unable to shed their exuvium and died. These results support the hypothesis that TcCP30 plays an integral role as a cross-linked structural protein in the formation of lightweight rigid cuticle of the beetle.
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