ArticleScientific reports2015
Oligonucleotide Probes for ND-FISH Analysis to Identify Rye and Wheat Chromosomes.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 101 papers.
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Who cites it
101 citing papers in PubMed.
- Characterization of Chromosomal Structural Polymorphism inPlants (Basel, Switzerland) · 2026Article
- Unveiling centromeric retrotransposon dynamics through a high-quality rye genome assembly.Nature communications · 2026Article
- Review
- Physical Mapping of a Powdery Mildew Resistance Gene in Chromosome 6St from Wheat-Plants (Basel, Switzerland) · 2026Article
- Characterization of a New Powdery Mildew Resistance Gene on Chromosome 1R from Hexaploid Triticale Transferred to Wheat.Plants (Basel, Switzerland) · 2026Article
- Using wheat-rye 1BL.1RS translocations to disclose the effects of subtelomeric tandem repeats on meiotic behavior of homologues.BMC plant biology · 2026Article
- Impact of Alien Chromosome Introgression fromPlants (Basel, Switzerland) · 2025Article
- Molecular characterization of a new wheat-Thinopyrum ponticum translocation line with resistance to stripe rust.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Characterization of a New Stripe Rust Resistance Gene on Chromosome 2StS fromPlants (Basel, Switzerland) · 2025Article
- Rapid isolation of Yr9 via MutIsoSeq and QTL analysis of durable stripe rust resistance in wheat cultivar Xingzi 9104.Stress biology · 2025Article
- Identification and Specific KASP Marker Development for Durum Wheat T2DS-2AS.2AL Translocation Line YL-429 with Wax Inhibitor GenePlants (Basel, Switzerland) · 2025Article
- Integrated review ofMolecular breeding : new strategies in plant improvement · 2025Review
- Establishment of a genome map-based karyotype ofFrontiers in plant science · 2025Article
- Article
- Stable minichromosome and functional neocentromere derived from rye 7R chromosome arm.BMC plant biology · 2024Article
- ND-FISH with New Oligo Probes for Chromosome Identification ofPlants (Basel, Switzerland) · 2024Article
- Characterization of New Wheat-Plants (Basel, Switzerland) · 2024Article
- Genome-wide simple sequence repeat analysis and specific molecular marker development of rye.BMC genomics · 2024Article
- A kinase fusion protein from Aegilops longissima confers resistance to wheat powdery mildew.Nature communications · 2024Article
- Article
41 more citing papers are in PubMed but not listed here.
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9 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Genomic in situ hybridization (GISH) has been widely used to detect rye (Secale cereale L.) chromosomes in wheat (Triticum aestivum L.) introgression lines. The routine procedure of GISH using genomic DNA of rye as a probe is time-consuming and labor-intensive because of the preparation and labeling of genomic DNA of rye and denaturing of chromosomes and probes. In this study, new oligonucleotide probes Oligo-1162, Oligo-pSc200 and Oligo-pSc250 were developed. The three new probes can be used for non-denaturing fluorescence in situ hybridization (ND-FISH) assays and replace genomic DNA of rye as a probe to discriminate rye chromosomes in wheat backgrounds. In addition, previously developed oligonucleotide probes Oligo-pSc119.2-1, Oligo-pSc119.2-2, Oligo-pTa535-1, Oligo-pTa535-2, Oligo-pTa71-2, Oligo-pAWRC.1 and Oligo-CCS1 can also be used for ND-FISH of wheat and rye. These probes have provided an easier, faster and more cost-effective method for the FISH analysis of wheat and hybrids derived from wheat × rye.
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