ArticleJournal of visualized experiments : JoVE2015
Applying stereotactic injection technique to study genetic effects on animal behaviors.
Article in Journal of visualized experiments : JoVE, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- CircFRRS1 drives neuroinflammation through the miR-27a-3p/TLR4 pathway after deep hypothermic circulatory arrest.Frontiers in cellular neuroscience · 2026Article
- Development and characterisation of improved unifocal primary mouse lung cancer models with metastatic potential.The Journal of pathology · 2025Article
- Stable, neuron-specific gene expression in the mouse brain.Journal of biological engineering · 2024Article
- Continued P2X7 activation leads to mitochondrial fission and compromising microglial phagocytosis after subarachnoid haemorrhage.Journal of neurochemistry · 2022Article
- Adaptation of prelimbic cortex mediated by IL-6/STAT3/Acp5 pathway contributes to the comorbidity of neuropathic pain and depression in rats.Journal of neuroinflammation · 2022Article
- Two photon imaging of calcium responses in murine Purkinje neurons.STAR protocols · 2022Article
- Modified Protocol for Establishment of Intracranial Arterial Dolichoectasia Model by Injection of Elastase Into Cerebellomedullary Cistern in Mice.Frontiers in neurology · 2022Article
- Downregulation of miR-383 reduces depression-like behavior through targeting Wnt family member 2 (Wnt2) in rats.Scientific reports · 2021Article
- Article
- A New Tool for the Analysis of the Effect of Intracerebrally Injected Anti-Amyloid-β Compounds.Journal of Alzheimer's disease : JAD · 2021Article
- Adaptable Angled Stereotactic Approach for Versatile Neuroscience Techniques.Journal of visualized experiments : JoVE · 2020Article
- Therapeutic effect of transplantation of human bone marrow‑derived mesenchymal stem cells on neuron regeneration in a rat model of middle cerebral artery occlusion.Molecular medicine reports · 2019Article
- DREADD in parvalbumin interneurons of the dentate gyrus modulates anxiety, social interaction and memory extinction.Current molecular medicine · 2016Article
Corrections and comments
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Authors and funding
2 authors.
Funding
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Abstract
Stereotactic injection is a useful technique to deliver high titer lentiviruses to targeted brain areas in mice. Lentiviruses can either overexpress or knockdown gene expression in a relatively focused region without significant damage to the brain tissue. After recovery, the injected mouse can be tested on various behavioral tasks such as the Open Field Test (OFT) and the Forced Swim Test (FST). The OFT is designed to assess locomotion and the anxious phenotype in mice by measuring the amount of time that a mouse spends in the center of a novel open field. A more anxious mouse will spend significantly less time in the center of the novel field compared to controls. The FST assesses the anti-depressive phenotype by quantifying the amount of time that mice spend immobile when placed into a bucket of water. A mouse with an anti-depressive phenotype will spend significantly less time immobile compared to control animals. The goal of this protocol is to use the stereotactic injection of a lentivirus in conjunction with behavioral tests to assess how genetic factors modulate animal behaviors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.