ArticlePloS one2015
The use of wavelength modulated Raman spectroscopy in label-free identification of T lymphocyte subsets, natural killer cells and dendritic cells.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed.
- Raman spectroscopy combined with multiple technologies for label-free identification of immune cells: An overview.Journal of pharmaceutical analysis · 2026Review
- Current Trends in In Vitro Diagnostics Using Surface-Enhanced Raman Scattering in Translational Biomedical Research.Biosensors · 2025Review
- Label-free ghost cytometry for manufacturing of cell therapy products.Scientific reports · 2024Article
- Raman Spectroscopy of Optically Trapped Living Human T Cell Subsets and Monocytes.International journal of molecular sciences · 2024Article
- Raman Spectroscopy as a Research and Diagnostic Tool in Clinical Hematology and Hematooncology.International journal of molecular sciences · 2024Review
- Advancing precision cancer immunotherapy drug development, administration, and response prediction with AI-enabled Raman spectroscopy.Frontiers in immunology · 2024Review
- Label-Free Characterization of Macrophage Polarization Using Raman Spectroscopy.International journal of molecular sciences · 2023Article
- Raman-based spectrophenotyping of the most important cells of the immune system.Journal of advanced research · 2022Article
- Identification of the Differentiation Stages of Living Cells from the Six Most Immature Murine Hematopoietic Cell Populations by Multivariate Analysis of Single-Cell Raman Spectra.Analytical chemistry · 2022Article
- Non-Invasive Three-Dimensional Cell Analysis in Bioinks by Raman Imaging.ACS applied materials & interfaces · 2022Article
- In vivo optical molecular imaging of inflammation and immunity.Journal of molecular medicine (Berlin, Germany) · 2021Review
- Biochemical Analysis of Leukocytes after In Vitro and In Vivo Activation with Bacterial and Fungal Pathogens Using Raman Spectroscopy.International journal of molecular sciences · 2021Article
- Non-invasive cell classification using the Paint Raman Express Spectroscopy System (PRESS).Scientific reports · 2021Article
- Lymphocyte expansion in bioreactors: upgrading adoptive cell therapy.Journal of biological engineering · 2021Review
- Vibrational Spectroscopy for In Vitro Monitoring Stem Cell Differentiation.Molecules (Basel, Switzerland) · 2020Review
- Real-time monitoring of live mycobacteria with a microfluidic acoustic-Raman platform.Communications biology · 2020Article
- Tracing Hematopoietic Progenitor Cell Neutrophilic Differentiation via Raman Spectroscopy.Bioconjugate chemistry · 2018Article
- Towards automated cancer screening: Label-free classification of fixed cell samples using wavelength modulated Raman spectroscopy.Journal of biophotonics · 2018Article
- Label-free optical vibrational spectroscopy to detect the metabolic state of M. tuberculosis cells at the site of disease.Scientific reports · 2017Article
- Raman Plus X: Biomedical Applications of Multimodal Raman Spectroscopy.Sensors (Basel, Switzerland) · 2017Review
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Determining the identity of cells of the immune system usually involves destructive fixation and chemical staining, or labeling with fluorescently labeled antibodies recognising specific cell surface markers. Completely label-free identification would be a significant advantage in conditions where untouched cells are a priority. We demonstrate here the use of Wavelength Modulated Raman Spectroscopy, to achieve label-free identification of purified, unfixed and untouched populations of major immune cell subsets isolated from healthy human donors. Using this technique we have been able to distinguish between CD4(+) T lymphocytes, CD8(+) T lymphocytes and CD56(+) Natural Killer cells at specificities of up to 96%. Additionally, we have been able to distinguish between CD303(+) plasmacytoid and CD1c(+) myeloid dendritic cell subsets, the key initiator and regulatory cells of many immune responses. This demonstrates the ability to identify unperturbed cells of the immune system, and opens novel opportunities to analyse immunological systems and to develop fully label-free diagnostic technologies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.