ArticlePloS one2015
Isolation of Enteric Nervous System Progenitor Cells from the Aganglionic Gut of Patients with Hirschsprung's Disease.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 46 citations in OpenAlex.
- Embryonic Gut Enteric Neural Crest Cells Derived Neurospheres.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Cecal growth factors promote enteric neurosphere formation and hindgut colonization in the avian model.Frontiers in cell and developmental biology · 2025Article
- Cutting-edge regenerative therapy for Hirschsprung disease and its allied disorders.Surgery today · 2024Review
- Agrin Inhibition in Enteric Neural Stem Cells Enhances Their Migration Following Colonic Transplantation.Stem cells translational medicine · 2024Article
- Intra-arterial delivery of neurospheres into isolated perfused porcine colons: a proof of concept.Biology methods & protocols · 2024Article
- Essential Role of BMP4 Signaling in the Avian Ceca in Colorectal Enteric Nervous System Development.International journal of molecular sciences · 2023Article
- Harnessing the Power of Enteric Glial Cells' Plasticity and Multipotency for Advancing Regenerative Medicine.International journal of molecular sciences · 2023Review
- A combinatorial panel for flow cytometry-based isolation of enteric nervous system cells from human intestine.EMBO reports · 2023Article
- Stem cell derived therapies to preserve and repair the developing intestine.Seminars in perinatology · 2023Article
- Schwann Cells in the Aganglionic Colon of Hirschsprung Disease Can Generate Neurons for Regenerative Therapy.Stem cells translational medicine · 2022Article
- Human Pluripotent Stem Cell-Based Models for Hirschsprung Disease: From 2-D Cell to 3-D Organoid Model.Cells · 2022Review
- Premigratory neural crest stem cells generate enteric neurons populating the mouse colon and regulating peristalsis in tissue-engineered intestine.Stem cells translational medicine · 2021Article
- Neuronal lineages derived from the nerve-associated Schwann cell precursors.Cellular and molecular life sciences : CMLS · 2021Review
- Comment on a recent genetic study on the risk profile of Hirschsprung's disease from a pediatric surgeon's perspective.Annals of translational medicine · 2020Article
- "Too much guts and not enough brains": (epi)genetic mechanisms and future therapies of Hirschsprung disease - a review.Clinical epigenetics · 2019Review
- Wnt Receptor Frizzled-4 as a Marker for Isolation of Enteric Neural Progenitors in Human Children.Cells · 2019Article
- DRG-Derived Neural Progenitors Differentiate into Functional Enteric Neurons Following Transplantation in the Postnatal Colon.Cell transplantation · 2019Article
- Recent developments in cell-based ENS regeneration - a short review.Innovative surgical sciences · 2018Review
- Neural Crest Cell Implantation Restores Enteric Nervous System Function and Alters the Gastrointestinal Transcriptome in Human Tissue-Engineered Small Intestine.Stem cell reports · 2017Article
- Postnatal human enteric neuronal progenitors can migrate, differentiate, and proliferate in embryonic and postnatal aganglionic gut environments.Pediatric research · 2017Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enteric nervous system progenitor cells isolated from postnatal human gut and cultured as neurospheres can then be transplanted into aganglionic gut to restore normal patterns of contractility. These progenitor cells may be of future use to treat patients with Hirschprung's disease, a congenital condition characterized by hindgut dysmotility due to the lack of enteric nervous system ganglia. Here we demonstrate that progenitor cells can also be isolated from aganglionic gut removed during corrective surgery for Hirschsprung's disease. Although the enteric nervous system marker calretinin is not expressed in the aganglionic gut region, de novo expression is initiated in cultured neurosphere cells isolated from aganglionic Hirschsprung bowel. Furthermore, expression of the neural markers NOS, VIP and GFAP also increased during culture of aganglionic gut neurospheres which we show can be transplantation into cultured embryonic mouse gut explants to restore a normal frequency of contractility. To determine the origin of the progenitor cells in aganglionic region, we used fluorescence-activated cell sorting to demonstrate that only p75-positive neural crest-derived cells present in the thickened nerve trunks characteristic of the aganglionic region of Hirschsprung gut gave rise to neurons in culture. The derivation of enteric nervous system progenitors in the aganglionic gut region of Hirschprung's patients not only means that this tissue is a potential source of cells for future autologous transplantation, but it also raises the possibility of inducing the differentiation of these endogenous cells in situ to compensate for the aganglionosis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.