ArticleBiophysical journal2015
Fluorescence lifetime imaging of membrane lipid order with a ratiometric fluorescent probe.
Article in Biophysical journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Microscopic insight into HIV fusion peptide-mediated dehydration and packing regulation in membranes.Biophysical journal · 2025Article
- HBimmCue: A Versatile Fluorescent Probe for Multi-Scale Imaging of Lipid Polarity and Membrane Order in Inner Mitochondrial Membrane.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Article
- Dysregulation of cellular membrane homeostasis as a crucial modulator of cancer risk.The FEBS journal · 2024Review
- Fluorescent Probes and Quenchers in Studies of Protein Folding and Protein-Lipid Interactions.Chemical record (New York, N.Y.) · 2024Review
- Imipramine Treatment Alters Sphingomyelin, Cholesterol, and Glycerophospholipid Metabolism in Isolated Macrophage Lysosomes.Biomolecules · 2023Article
- A molecular rotor FLIM probe reveals dynamic coupling between mitochondrial inner membrane fluidity and cellular respiration.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Cell density-dependent membrane distribution of ganglioside GM3 in melanoma cells.Cellular and molecular life sciences : CMLS · 2023Article
- Biocompatible and optically stable hydrophobic fluorescent carbon dots for isolation and imaging of lipid rafts in model membrane.Analytical and bioanalytical chemistry · 2022Article
- Plant Sterol Clustering Correlates with Membrane Microdomains as Revealed by Optical and Computational Microscopy.Membranes · 2021Article
- Fluorescence lifetime imaging microscopy and time-resolved anisotropy of nanomaterial-induced changes to red blood cell membranes.Methods and applications in fluorescence · 2021Article
- How Does Liquid-Liquid Phase Separation in Model Membranes Reflect Cell Membrane Heterogeneity?Membranes · 2021Review
- Imaging non-classical mechanical responses of lipid membranes using molecular rotors.Chemical science · 2020Article
- Effects of titanium dioxide and zinc oxide nano-materials on lipid order in model membranes.Biochimica et biophysica acta. Biomembranes · 2020Article
- PTEN activation contributes to neuronal and synaptic engulfment by microglia in tauopathy.Acta neuropathologica · 2020Article
- Lipid Rafts: Controversies Resolved, Mysteries Remain.Trends in cell biology · 2020Review
- Single-cell glycolytic activity regulates membrane tension and HIV-1 fusion.PLoS pathogens · 2020Article
- Liquid-Ordered Phase Formation by Mammalian and Yeast Sterols: A Common Feature With Organizational Differences.Frontiers in cell and developmental biology · 2020Article
- Lipid Bilayer Thickness Measured by Quantitative DIC Reveals Phase Transitions and Effects of Substrate Hydrophilicity.Langmuir : the ACS journal of surfaces and colloids · 2019Article
- Glycan-decorated protocells: novel features for rebuilding cellular processes.Interface focus · 2019Review
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To monitor the lateral segregation of lipids into liquid-ordered (Lo) and -disordered (Ld) phases in lipid membranes, environment-sensitive dyes that partition in both phases but stain them differently have been developed. Of particular interest is the dual-color F2N12S probe, which can discriminate the two phases through the ratio of its two emission bands. These bands are associated with the normal (N(∗)) and tautomer (T(∗)) excited-state species that result from an excited-state intramolecular proton transfer. In this work, we investigated the potency of the time-resolved fluorescence parameters of F2N12S to discriminate lipid phases in model and cell membranes. Both the long and mean lifetime values of the T(∗) form of F2N12S were found to differ by twofold between Ld and Lo phases as a result of the restriction in the relative motions of the two aromatic moieties of F2N12S imposed by the highly packed Lo phase. This differed from the changes in the ratio of the two emission bands between the two phases, which mainly resulted from the decreased hydration of the N(∗) form in the Lo phase. Importantly, the strong difference in lifetimes between the two phases was preserved when cholesterol was added to the Ld phase. The two phases could be imaged with high contrast by fluorescence lifetime imaging microscopy (FLIM) on giant unilamellar vesicles. FLIM images of F2N12S-labeled live HeLa cells confirmed that the plasma membrane was mainly in the Lo-like phase. Furthermore, the two phases were found to be homogeneously distributed all over the plasma membrane, indicating that they are highly mixed at the spatiotemporal resolution of the FLIM setup. Finally, FLIM could also be used to sensitively monitor the change in lipid phase upon cholesterol depletion and apoptosis.
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