ArticleBiophysical journal2015
Deuterium NMR of raft model membranes reveals domain-specific order profiles and compositional distribution.
Article in Biophysical journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Cholesterol in model membranes.Biophysical reviews · 2025Review
- Distinct effects of progesterone and cholesterol on lipid membranes: insights from biophysical experiments and molecular dynamics simulations.Frontiers in molecular biosciences · 2025Article
- Lipid Rafts in Signalling, Diseases, and Infections: What Can Be Learned from Fluorescence Techniques?Membranes · 2025Review
- Sterol-lipids enable large-scale, liquid-liquid phase separation in bilayer membranes of only two components.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Dehydration of Lipid Membranes Drives Redistribution of Cholesterol Between Lateral Domains.The journal of physical chemistry letters · 2024Article
- Biophysical studies of lipid nanodomains using different physical characterization techniques.Biophysical journal · 2023Review
- Effect of cholesterol on the lactosylceramide domains in phospholipid bilayers.Biophysical journal · 2022Article
- Amphidinol 3 preferentially binds to cholesterol in disordered domains and disrupts membrane phase separation.Biochemistry and biophysics reports · 2021Article
- Lateral heterogeneity and domain formation in cellular membranes.Chemistry and physics of lipids · 2020Article
- Sphingomyelins and ent-Sphingomyelins Form Homophilic Nano-Subdomains within Liquid Ordered Domains.Biophysical journal · 2020Article
- Assembly formation of minor dihydrosphingomyelin in sphingomyelin-rich ordered membrane domains.Scientific reports · 2020Article
- Serotonin Alters the Phase Equilibrium of a Ternary Mixture of Phospholipids and Cholesterol.Frontiers in physiology · 2020Article
- Normal vibrations of ternary DOPC/DPPC/cholesterol lipid bilayers by low-frequency Raman spectroscopy.RSC advances · 2019Article
- The Affinity of Sterols for Different Phospholipid Classes and Its Impact on Lateral Segregation.Biophysical journal · 2019Article
- Phospholipid Chain Interactions with Cholesterol Drive Domain Formation in Lipid Membranes.Biophysical journal · 2018Article
- Dynamic membrane interactions of antibacterial and antifungal biomolecules, and amyloid peptides, revealed by solid-state NMR spectroscopy.Biochimica et biophysica acta. General subjects · 2018Review
- DHA Modifies the Size and Composition of Raftlike Domains: A Solid-StateBiophysical journal · 2018Article
- Essential Insights into Lipid Membrane Organization from Essential Fatty Acids.Biophysical journal · 2018Article
- Emphatic visualization of sphingomyelin-rich domains by inter-lipid FRET imaging using fluorescent sphingomyelins.Scientific reports · 2017Article
- Bilayer Interactions among Unsaturated Phospholipids, Sterols, and Ceramide.Biophysical journal · 2017Article
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4 authors.
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Abstract
In this report, we applied site-specifically deuterated N-stearoylsphingomyelins (SSMs) to raft-exhibiting ternary mixtures containing SSM, 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), and cholesterol (Chol) and successfully acquired deuterium quadrupole coupling profiles of SSM from liquid-ordered (Lo) and liquid-disordered (Ld) domains. To our knowledge, this is the first report that shows detailed lipid chain dynamics separately and simultaneously obtained from coexisting Lo and Ld domains. We also found that the quadrupole profile of the Lo phase in the ternary system was almost identical to that in the SSM-Chol binary mixture, suggesting that the order profile of the binary system is essentially applicable to more complicated membrane systems in terms of the acyl chain order. We also demonstrated that (2)H NMR spectroscopy, in combination with organic synthesis of deuterated components, could be used to reveal the accurate mole fractions of each component distributed in the Lo and Ld domains. As compared with the reported tie-line analysis of phase diagrams, the merit of our (2)H NMR analysis is that the domain-specific compositional fractions are directly attainable without experimental complexity and ambiguity. The accurate compositional distributions as well as lipid order profiles in ternary mixtures are relevant to understanding the molecular mechanism of lipid raft formation.
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