ArticlePloS one2015
A Genetic Screen for Pathogenicity Genes in the Hemibiotrophic Fungus Colletotrichum higginsianum Identifies the Plasma Membrane Proton Pump Pma2 Required for Host Penetration.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 36 citations in OpenAlex.
- Transcriptomic and Metabolomic Analyses Reveal the Phenylpropanoid Metabolism Regulatory Network inLife (Basel, Switzerland) · 2026Article
- Breaking dependence on melanisation imparts diversity to a dogmatic invasion strategy of phytopathogenic fungi.Nature communications · 2026Article
- An Ammonium Transporter Gene Contributes to the Aggressiveness of the Dutch Elm Disease PathogenJournal of fungi (Basel, Switzerland) · 2026Article
- The Plasma Membrane HInternational journal of molecular sciences · 2023Article
- Two Novel Dimorphism-Related Virulence Factors ofInternational journal of molecular sciences · 2021Article
- An Efficient Strategy for Obtaining Mutants by Targeted Gene Deletion inFrontiers in microbiology · 2021Article
- Acetyl-coenzyme A synthetase gene ChAcs1 is essential for lipid metabolism, carbon utilization and virulence of the hemibiotrophic fungus Colletotrichum higginsianum.Molecular plant pathology · 2019Article
- Review
- Pleiotropic Roles of ChSat4 in Asexual Development, Cell Wall Integrity Maintenance, and Pathogenicity inFrontiers in microbiology · 2018Article
- A Dispensable Chromosome Is Required for Virulence in the Hemibiotrophic Plant PathogenFrontiers in microbiology · 2018Article
- Article
- How alkalinization drives fungal pathogenicity.PLoS pathogens · 2017Review
- Rapid Deletion Production in Fungi via Agrobacterium Mediated Transformation of OSCAR Deletion Constructs.Journal of visualized experiments : JoVE · 2017Article
- Rhamnose synthase activity is required for pathogenicity of the vascular wilt fungus Verticillium dahliae.Molecular plant pathology · 2017Article
- ChMob2 binds to ChCbk1 and promotes virulence and conidiation of the fungal pathogen Colletotrichum higginsianum.BMC microbiology · 2017Article
- A silver bullet in a golden age of functional genomics: the impact ofFungal biology and biotechnology · 2017Review
- Sugar Accumulation in Leaves of ArabidopsisFrontiers in plant science · 2017Article
- ChSte7 Is Required for Vegetative Growth and Various Plant Infection Processes in Colletotrichum higginsianum.BioMed research international · 2016Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We used insertional mutagenesis by Agrobacterium tumefaciens mediated transformation (ATMT) to isolate pathogenicity mutants of Colletotrichum higginsianum. From a collection of 7200 insertion mutants we isolated 75 mutants with reduced symptoms. 19 of these were affected in host penetration, while 17 were affected in later stages of infection, like switching to necrotrophic growth. For 16 mutants the location of T-DNA insertions could be identified by PCR. A potential plasma membrane H(+)-ATPase Pma2 was targeted in five independent insertion mutants. We genetically inactivated the Ku80 component of the non-homologous end-joining pathway in C. higginsianum to establish an efficient gene knockout protocol. Chpma2 deletion mutants generated by homologous recombination in the ΔChku80 background form fully melanized appressoria but entirely fail to penetrate the host tissue and are non-pathogenic. The ChPMA2 gene is induced upon appressoria formation and infection of A. thaliana. Pma2 activity is not important for vegetative growth of saprophytically growing mycelium, since the mutant shows no growth penalty under these conditions. Colletotrichum higginsianum codes for a closely related gene (ChPMA1), which is highly expressed under most growth conditions. ChPMA1 is more similar to the homologous yeast genes for plasma membrane pumps. We propose that expression of a specific proton pump early during infection may be common to many appressoria forming fungal pathogens as we found ChPMA2 orthologs in several plant pathogenic fungi.
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