ArticleScientific reports2015
Effects and Mechanism of Atmospheric-Pressure Dielectric Barrier Discharge Cold Plasma on Lactate Dehydrogenase (LDH) Enzyme.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 155 citations in OpenAlex.
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- Identification of Different Classes of VOCs Based on Optical Emission Spectra Using a Dielectric Barrier Helium Plasma Coupled with a Mini Spectrometer.ACS measurement science au · 2024Article
- Immobilization protects enzymes from plasma-mediated inactivation.Journal of the Royal Society, Interface · 2023Article
- Mechanisms of bacterial inhibition and tolerance around cold atmospheric plasma.Applied microbiology and biotechnology · 2023Review
- Current and Potential Applications of Atmospheric Cold Plasma in the Food Industry.Molecules (Basel, Switzerland) · 2023Review
- An omics approach to delineating the molecular mechanisms that underlie the biological effects of physical plasma.Biophysics reviews · 2023Review
- Inactivation of Soybean Trypsin Inhibitor by Dielectric-Barrier Discharge Plasma and Its Safety Evaluation and Application.Foods (Basel, Switzerland) · 2022Article
- Effects of non-thermal atmospheric plasma on protein.Journal of clinical biochemistry and nutrition · 2022Article
- Liquid-type plasma-controlled in situ crosslinking of silk-alginate injectable gel displayed better bioactivities and mechanical properties.Materials today. Bio · 2022Article
- Changes in Biochemical Properties and Activity of Trypsin-like Protease (Foods (Basel, Switzerland) · 2022Article
- Investigation on the impact of quality characteristics and storage stability of foxtail millet induced by air cold plasma.Frontiers in nutrition · 2022Article
- Effects of Atmospheric Plasma Corona Discharge onMicroorganisms · 2021Article
- An Application of Cold Atmospheric Plasma to Enhance Physiological and Biochemical Traits of Basil.Plants (Basel, Switzerland) · 2021Article
- Impact of Reactive Oxygen and Nitrogen Species Produced by Plasma on Mdm2-p53 Complex.International journal of molecular sciences · 2021Article
- Role of Short- and Long-Lived Reactive Species on the Selectivity and Anti-Cancer Action of Plasma Treatment In Vitro.Cancers · 2021Article
- Effects of Atmospheric Plasma Corona Discharges on Soil Bacteria Viability.Microorganisms · 2020Article
- Plasma-Driven in Situ Production of Hydrogen Peroxide for Biocatalysis.ChemSusChem · 2020Article
- Study on Chemical Modifications of Glutathione by Cold Atmospheric Pressure Plasma (Cap) Operated in Air in the Presence of Fe(II) and Fe(III) Complexes.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Proteins are carriers of biological functions and the effects of atmospheric-pressure non-thermal plasmas on proteins are important to applications such as sterilization and plasma-induced apoptosis of cancer cells. Herein, we report our detailed investigation of the effects of helium-oxygen non-thermal dielectric barrier discharge (DBD) plasmas on the inactivation of lactate dehydrogenase (LDH) enzyme solutions. Circular dichroism (CD) and dynamic light scattering (DLS) indicate that the loss of activity stems from plasma-induced modification of the secondary molecular structure as well as polymerization of the peptide chains. Raising the treatment intensity leads to a reduced alpha-helix content, increase in the percentage of the beta-sheet regions and random sequence, as well as gradually decreasing LDH activity. However, the structure of the LDH plasma-treated for 300 seconds exhibits a recovery trend after storage for 24 h and its activity also increases slightly. By comparing direct and indirect plasma treatments, plasma-induced LDH inactivation can be attributed to reactive species (RS) in the plasma, especially ones with a long lifetime including hydrogen peroxide, ozone, and nitrate ion which play the major role in the alteration of the macromolecular structure and molecular diameter in lieu of heat, UV radiation, and charged particles.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.