ArticleNucleic acids research2015
ATP-dependent G-quadruplex unfolding by Bloom helicase exhibits low processivity.
Article in Nucleic acids research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 43 citations in OpenAlex.
- How DNA secondary structures drive replication fork instability.DNA repair · 2025Review
- Structure, Topology, and Stability of Multiple G-quadruplexes in Long Telomeric Overhangs.Journal of molecular biology · 2024Review
- Shelterin reduces the accessibility of telomeric overhangs.Nucleic acids research · 2022Article
- Emerging accessibility patterns in long telomeric overhangs.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Remodeling the conformational dynamics of I-motif DNA by helicases in ATP-independent mode at acidic environment.iScience · 2022Article
- Fast interaction dynamics of G-quadruplex and RGG-rich peptides unveiled in zero-mode waveguides.Nucleic acids research · 2021Article
- Characterization of G-Quadruplexes Folding/Unfolding Dynamics and Interactions with Proteins from Single-Molecule Force Spectroscopy.Biomolecules · 2021Review
- HO-1 and Heme: G-Quadruplex Interaction Choreograph DNA Damage Responses and Cancer Growth.Cells · 2021Review
- Targeting G-quadruplex Forming Sequences with Cas9.ACS chemical biology · 2021Article
- Interrogating accessibility of telomeric sequences with FRET-PAINT: evidence for length-dependent telomere compaction.Nucleic acids research · 2021Article
- Single-molecule visualization of human BLM helicase as it acts upon double- and single-stranded DNA substrates.Nucleic acids research · 2019Article
- Quantifying the impact of small molecule ligands on G-quadruplex stability against Bloom helicase.Nucleic acids research · 2019Article
- Replication of G Quadruplex DNA.Genes · 2019Review
- Single molecule kinetics uncover roles for E. coli RecQ DNA helicase domains and interaction with SSB.Nucleic acids research · 2018Article
- A practical guide to studying G-quadruplex structures using single-molecule FRET.Molecular genetics and genomics : MGG · 2017Review
- A single molecule study of a fluorescently labeled telomestatin derivative and G-quadruplex interactions.Nucleic acids research · 2017Article
- Single-molecule imaging reveals a common mechanism shared by G-quadruplex-resolving helicases.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- A Comparative Study of G-Quadruplex Unfolding and DNA Reeling Activities of Human RECQ5 Helicase.Biophysical journal · 2016Article
- G-quadruplexes and helicases.Nucleic acids research · 2016Review
- Hypothesis: Paralog Formation from Progenitor Proteins and Paralog Mutagenesis Spur the Rapid Evolution of Telomere Binding Proteins.Frontiers in genetics · 2016Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Various helicases and single stranded DNA (ssDNA) binding proteins unfold G-quadruplex (GQ) structures. However, the underlying mechanisms of this activity have only recently come to focus. We report kinetic studies on Bloom (BLM) helicase and human telomeric GQ interactions using single-molecule Förster resonance energy transfer (smFRET). Using partial duplex DNA (pdDNA) constructs with different 5' ssDNA overhangs, we show that BLM localizes in the vicinity of ssDNA/double-stranded DNA (dsDNA) junction and reels in the ssDNA overhang in an ATP-dependent manner. A comparison of DNA constructs with or without GQ in the overhang shows that GQ unfolding is achieved in 50-70% of reeling attempts under physiological salt and pH conditions. The unsuccessful attempts often result in dissociation of BLM from DNA which slows down the overall BLM activity. BLM-mediated GQ unfolding is typically followed by refolding of the GQ, a pattern that is repeated several times before BLM dissociates from DNA. BLM is significantly less processive compared to the highly efficient GQ destabilizer Pif1 that can repeat GQ unfolding activity hundreds of times before dissociating from DNA. Despite the variations in processivity, our studies point to possible common patterns used by different helicases in minimizing the duration of stable GQ formation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.