ArticleNucleic acids research2015
Rational evolution of Cd2+-specific DNAzymes with phosphorothioate modified cleavage junction and Cd2+ sensing.
Article in Nucleic acids research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed.
- Chemically modified DNA aptamers and DNAzymes for expanded functional capabilities.Chemical science · 2026Review
- Signal Transduction Strategies for DNAzyme-Based Sensing and Imaging of Metal Ions in Cells andChemical & biomedical imaging · 2025Review
- On-Site Portable Lithium Detection in Mining and Recycling Industries Based on a DNAzyme Fluorescent Sensor.Angewandte Chemie (International ed. in English) · 2025Article
- Harnessing sulfur-binding domains to separate Sp and Rp isomers of phosphorothioate oligonucleotides.Applied microbiology and biotechnology · 2024Article
- Hybrid Nanoparticle/DNAzyme Electrochemical Biosensor for the Detection of Divalent Heavy Metal Ions and CrSensors (Basel, Switzerland) · 2023Article
- A Semisynthetic Bioluminescence Sensor for Ratiometric Imaging of Metal Ions In Vivo Using DNAzymes Conjugated to An Engineered Nano-Luciferase.Angewandte Chemie (International ed. in English) · 2023Article
- Ratiometric Detection of ZnChemistry (Basel, Switzerland) · 2023Article
- A Highly Selective Mn(II)-Specific DNAzyme and Its Application in Intracellular Sensing.JACS Au · 2023Article
- Recent Aptamer-Based Biosensors for CdBiosensors · 2023Review
- Simultaneous FeScience advances · 2023Article
- Monitoring leaching of CdBiosensors & bioelectronics · 2023Article
- A Sensitive Aptamer Fluorescence Anisotropy Sensor for CdBiosensors · 2022Article
- Development of a DNAzyme-based colorimetric biosensor assay for dual detection of CdAnalytical and bioanalytical chemistry · 2021Article
- Recent Advances on Functional Nucleic-Acid Biosensors.Sensors (Basel, Switzerland) · 2021Review
- Biosensing with DNAzymes.Chemical Society reviews · 2021Review
- Nucleic Acids Analysis.Science China. Chemistry · 2021Review
- Modified Nucleic Acids: Expanding the Capabilities of Functional Oligonucleotides.Molecules (Basel, Switzerland) · 2020Review
- In vitro Selection of Chemically Modified DNAzymes.ChemistryOpen · 2020Review
- The L-platform/L-scaffold framework: a blueprint for RNA-cleaving nucleic acid enzyme design.RNA (New York, N.Y.) · 2020Article
- Catalytic Nucleic Acids: Biochemistry, Chemical Biology, Biosensors, and Nanotechnology.iScience · 2020Review
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2 authors.
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Abstract
In vitro selection of RNA-cleaving DNAzymes is a powerful method for isolating metal-specific DNA. A few successful examples are known, but it is still difficult to target some thiophilic metals such as Cd(2+) due to limited functional groups in DNA. While using modified bases expands the chemical functionality of DNA, a single phosphorothioate modification might boost its affinity for thiophilic metals without complicating the selection process or using bases that are not commercially available. In this work, the first such in vitro selection for Cd(2+) is reported. After using a blocking DNA and negative selections to rationally direct the library outcome, a highly specific DNAzyme with only 12 nucleotides in the catalytic loop is isolated. This DNAzyme has a cleavage rate of 0.12 min(-1) with 10 μM Cd(2+) at pH 6.0. The Rp form of the substrate is cleaved ∼100-fold faster than the Sp form. The DNAzyme is most active with Cd(2+) and its selectivity against Zn(2+) is over 100 000-fold. Its application in detecting Cd(2+) is also demonstrated. The idea of introducing single modifications in the fixed region expands the scope of DNA/metal interactions with minimal perturbation of DNA structure and property.
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