Evidence map›Paper›PMID 25990244›Full record

ArticleJournal of leukocyte biology2015

Low DICER1 expression is associated with attenuated neutrophil differentiation and autophagy of NB4 APL cells.

Julian Wampfler, Elena A Federzoni, Bruce E Torbett, Martin F Fey, Mario P Tschan

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of leukocyte biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.6field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Article
  5. Article
  6. Autophagy-Regulating microRNAs and Cancer.Frontiers in oncology · 2017
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 2 countries.

Julian Wampfler*Division of Experimental Pathology, Institute of Pathology, and Graduate School for Cellular and Biomedical Sciences, University of Bern, Switzerland; Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California, USA; and Department of Medical Oncology, Inselspital, Bern University Hospital, Bern, Switzerland.
Elena A Federzoni*Division of Experimental Pathology, Institute of Pathology, and Graduate School for Cellular and Biomedical Sciences, University of Bern, Switzerland; Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California, USA; and Department of Medical Oncology, Inselspital, Bern University Hospital, Bern, Switzerland.
Bruce E Torbett*Division of Experimental Pathology, Institute of Pathology, and Graduate School for Cellular and Biomedical Sciences, University of Bern, Switzerland; Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California, USA; and Department of Medical Oncology, Inselspital, Bern University Hospital, Bern, Switzerland.
Martin F Fey*Division of Experimental Pathology, Institute of Pathology, and Graduate School for Cellular and Biomedical Sciences, University of Bern, Switzerland; Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California, USA; and Department of Medical Oncology, Inselspital, Bern University Hospital, Bern, Switzerland.
Mario P Tschan*Division of Experimental Pathology, Institute of Pathology, and Graduate School for Cellular and Biomedical Sciences, University of Bern, Switzerland; Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California, USA; and Department of Medical Oncology, Inselspital, Bern University Hospital, Bern, Switzerland mario.tschan@pathology.unibe.ch.
Scripps Research Institute · USUniversity of Bern · CHUniversity Hospital of Bern · CH

Funding

iPSC-based blood regenerative therapies for AIDSR01HL116221 · NHLBI · UNIVERSITY OF WISCONSIN-MADISON · PI SLUKVIN, IGOR I., TORBETT, BRUCE EDWARD · 2013 to 2017
$3.4M
NHLBI NIH HHS 1R01HL116221-01NHLBI NIH HHS R01 HL116221
6 · The paper itself

Abstract

Successful myeloid differentiation depends on the expression of a series of miRNAs. Thus, it is hardly surprising that miRNAs are globally repressed in AML, a disease mainly characterized by a block in cellular myeloid differentiation. Studies investigating the mechanisms for low miRNA expression in AML has mostly focused on altered transcriptional regulation or deletions, whereas defective miRNA processing has received less attention. In this study, we report that the expression of the key miRNA processing enzyme DICER1 is down-regulated in primary AML patient samples and healthy CD34(+) progenitor cells as compared with granulocytes. In line with these findings, Dicer1 expression was induced significantly in AML cell lines upon neutrophil differentiation. The knocking down of DICER1 in AML cells significantly attenuated neutrophil differentiation, which was paralleled by decreased expression of miRNAs involved in this process. Moreover, we found that inhibiting DICER1 attenuated the activation of autophagy, a cellular recycling process that is needed for proper neutrophil differentiation of AML cells. Our results clearly indicate that DICER1 plays a novel role in neutrophil differentiation as well as in myeloid autophagy of AML cells.

Indexed as

AutophagyCell DifferentiationCell Line, TumorDEAD-box RNA HelicasesGene Knockdown TechniquesHumansLeukemia, Promyelocytic, AcuteMicroRNAsNeutrophilsRibonuclease IIIRNA, MessengerRNA Processing, Post-TranscriptionalTretinoinDEAD-box RNA HelicasesDICER1 protein, humanMicroRNAsRibonuclease IIIRNA, MessengerTretinoinAMLprocessing machineryRNAi

Identifiers

PMID25990244
PMCPMC4541502
OpenAlexW2161547923

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.