ArticleParasitology research2015
Molecular identification, immunolocalization, and characterization of Clonorchis sinensis triosephosphate isomerase.
Article in Parasitology research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 13 citations in OpenAlex.
- iTRAQ-based comparative proteomic analysis in different developmental stages of Echinococcus granulosus.Parasite (Paris, France) · 2021Article
- Structural Analysis of an Epitope Candidate of Triosephosphate Isomerase in Opisthorchis viverrini.Scientific reports · 2018Article
- Molecular characterization of triosephosphate isomerase from Echinococcus granulosus.Parasitology research · 2018Article
- Adaptation of the secretome of Echinostoma caproni may contribute to parasite survival in a Th1 milieu.Parasitology research · 2018Article
- Proteomic profile of the Bradysia odoriphaga in response to the microbial secondary metabolite benzothiazole.Scientific reports · 2016Article
- In silico cloning and B/T cell epitope prediction of triosephosphate isomerase from Echinococcus granulosus.Parasitology research · 2016Article
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Clonorchis sinensis triosephosphate isomerase (CsTIM) is a key regulatory enzyme of glycolysis and gluconeogenesis, which catalyzes the interconversion of glyceraldehyde 3-phosphate to dihydroxyacetone phosphate. In this study, the biochemical characterizations of CsTIM have been examined. A full-length complementary DNA (cDNA; Cs105350) sequence encoding CsTIM was obtained from our C. sinensis cDNA library. The open reading frame of CsTIM contains 759 bp which encodes 252 amino acids. The amino acid sequence of CsTIM shares 60-65% identity with other species. Western blot analysis displayed that recombinant CsTIM (rCsTIM) can be probed by anti-rCsTIM rat serum and anti-C. sinensis excretory/secretory products (anti-CsESPs) rat serum. Quantitative reverse transcription (RT)-PCR and western blotting analysis revealed that CsTIM messenger RNA (mRNA) and protein were differentially expressed in development cycle stages of the parasite, including adult worm, metacercaria, excysted metacercaria, and egg. In addition, immunolocalization assay showed that CsTIM was located in the seminal vesicle, eggs, and testicle. Moreover, rCsTIM exhibited active enzyme activity in catalytic reactions. The Michaelis constant (K m) of rCsTIM was 0.33 mM, when using glyceraldehyde 3-phosphate as the substrate. The optimal temperature and pH of CsTIM were 37 °C and 7.5-9.5, respectively. Collectively, these results suggest that CsTIM is an important protein involved in glycometabolism, and CsTIM possibly take part in many biological functions in the growth and development of C. sinensis.
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