ArticleScientific reports2015
The Galvanotactic Migration of Keratinocytes is Enhanced by Hypoxic Preconditioning.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- A multi therapy bioelectronic wound dressing.npj biomedical innovations · 2026Article
- Piezoelectric and Triboelectric Nanogenerators for Enhanced Wound Healing.Biomimetics (Basel, Switzerland) · 2023Review
- The Epidermal Keratinocyte as a Therapeutic Target for Management of Diabetic Wounds.International journal of molecular sciences · 2023Review
- Electric field promotes dermal fibroblast transdifferentiation through activation of RhoA/ROCK1 pathway.International journal of medical sciences · 2023Article
- ROS Promote Hypoxia-Induced Keratinocyte Epithelial-Mesenchymal Transition by Inducing SOX2 Expression and Subsequent Activation of Wnt/Oxidative medicine and cellular longevity · 2022Article
- Electric Factors in Wound Healing.Advances in wound care · 2021Article
- Overriding native cell coordination enhances external programming of collective cell migration.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Electric field stimulation for tissue engineering applications.BMC biomedical engineering · 2021Review
- Biomedical applications of electrical stimulation.Cellular and molecular life sciences : CMLS · 2020Review
- SCHEEPDOG: Programming Electric Cues to Dynamically Herd Large-Scale Cell Migration.Cell systems · 2020Article
- Real-time physiological measurements of oxygen using a non-invasive self-referencing optical fiber microsensor.Nature protocols · 2020Article
- Electric field down-regulates CD9 to promote keratinocytes migration through AMPK pathway.International journal of medical sciences · 2020Article
- Involvement of autophagy in hypoxia-BNIP3 signaling to promote epidermal keratinocyte migration.Cell death & disease · 2019Article
- High Glucose Suppresses Keratinocyte Migration Through the Inhibition of p38 MAPK/Autophagy Pathway.Frontiers in physiology · 2019Article
- Early redox activities modulate Xenopus tail regeneration.Nature communications · 2018Article
- Electrically stimulated cell migration and its contribution to wound healing.Burns & trauma · 2018Review
- Low intensity transcranial electric stimulation: Safety, ethical, legal regulatory and application guidelines.Clinical neurophysiology : official journal of the International Federation of Clinical Neurophysiology · 2017Review
- Notch1 Signaling Contributes to Hypoxia-induced High Expression of Integrin β1 in Keratinocyte Migration.Scientific reports · 2017Article
- Uniform electric field generation in circular multi-well culture plates using polymeric inserts.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The endogenous electric field (EF)-directed migration of keratinocytes (galvanotaxis) into wounds is an essential step in wound re-epithelialization. Hypoxia, which occurs immediately after injury, acts as an early stimulus to initiate the healing process; however, the mechanisms for this effect, remain elusive. We show here that the galvanotactic migration of keratinocytes was enhanced by hypoxia preconditioning as a result of the increased directionality rather than the increased motility of keratinocytes. This enhancement was both oxygen tension- and preconditioning time-dependent, with the maximum effects achieved using 2% O2 preconditioning for 6 hours. Hypoxic preconditioning (2% O2, 6 hours) decreased the threshold voltage of galvanotaxis to < 25 mV/mm, whereas this value was between 25 and 50 mV/mm in the normal culture control. In a scratch-wound monolayer assay in which the applied EF was in the default healing direction, hypoxic preconditioning accelerated healing by 1.38-fold compared with the control conditions. Scavenging of the induced ROS by N-acetylcysteine (NAC) abolished the enhanced galvanotaxis and the accelerated healing by hypoxic preconditioning. Our data demonstrate a novel and unsuspected role of hypoxia in supporting keratinocyte galvanotaxis. Enhancing the galvanotactic response of cells might therefore be a clinically attractive approach to induce improved wound healing.
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