ReviewFrontiers in molecular biosciences2014
Temporal resolution in fluorescence imaging.
Review in Frontiers in molecular biosciences, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Temporally resolved SMLM (with large PAR shift) enabled visualization of dynamic HA cluster formation and migration in a live cell.Scientific reports · 2023Article
- Miniaturized structured illumination microscopy using two 3-axis MEMS micromirrors.Biomedical optics express · 2022Article
- Through the Eyes of Creators: Observing Artificial Molecular Motors.ACS nanoscience Au · 2022Review
- Challenges and opportunities for small volumes delivery into the skin.Biomicrofluidics · 2021Article
- Imaging the Neuroimmune Dynamics Across Space and Time.Frontiers in neuroscience · 2020Review
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Authors and funding
1 author.
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Abstract
Temporal resolution is a key factor for imaging rapidly occurring events in biology. In this feature article, I investigate an approximate estimate for determining the temporal resolution limit. The condition that led to this limit is, the time taken by the ensemble (99.9%) of excited molecules to relax to ground state, assuming all the emitted photons are detected. In a simplistic three-level system, the temporal resolution is, ≈3τ p , where τ p = (log e 10)/(kf + knr ) and, kf and knr are respectively the radiative and non-radiative emission rates. This further assumes the ideal condition that, the quantum efficiency of the detector is unity and there are no other loses. We discuss few state-of-art microscopy techniques that are capable of high temporal resolution. This includes techniques such as multifocal multiphoton microscopy (MMM), multifocal plane microscopy, multiple excitation spot optical microscopy (MESO), multiplane microscopy and multiple light-sheet microscopy (MLSM).
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