ArticlePerspectives in medicinal chemistry2015
Applications of second-harmonic generation imaging microscopy in ovarian and breast cancer.
Article in Perspectives in medicinal chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 87 citations in OpenAlex.
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- Quantitative Histopathology Analysis Based on Label-free Multiphoton Imaging for Breast Cancer Diagnosis and Neoadjuvant Immunotherapy Response Assessment.International journal of biological sciences · 2025Article
- Machine learning for automated classification of lung collagen in a urethane-induced lung injury mouse model.Biomedical optics express · 2024Article
- Imaging at the nexus: how state of the art imaging techniques can enhance our understanding of cancer and fibrosis.Journal of translational medicine · 2024Review
- Review
- Leveraging mid-infrared spectroscopic imaging and deep learning for tissue subtype classification in ovarian cancer.The Analyst · 2023Article
- Exploration of utility of combined optical photothermal infrared and Raman imaging for investigating the chemical composition of microcalcifications in breast cancer.Analytical methods : advancing methods and applications · 2023Article
- Deep learning-based high-speed, large-field, and high-resolution multiphoton imaging.Biomedical optics express · 2023Article
- Types of spectroscopy and microscopy techniques for cancer diagnosis: a review.Lasers in medical science · 2022Review
- PSHG-TISS: A collection of polarization-resolved second harmonic generation microscopy images of fixed tissues.Scientific data · 2022Article
- Second-Harmonic Generation Imaging Reveals Changes in Breast Tumor Collagen Induced by Neoadjuvant Chemotherapy.Cancers · 2022Article
- Advances in nonlinear optical microscopy techniques for in vivo and in vitro neuroimaging.Biophysical reviews · 2021Review
- Influence of hematoxylin and eosin staining on the quantitative analysis of second harmonic generation imaging of fixed tissue sections.Biomedical optics express · 2021Article
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2 authors at 1 institution in 1 country.
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Abstract
In this perspective, we discuss how the nonlinear optical technique of second-harmonic generation (SHG) microscopy has been used to greatly enhance our understanding of the tumor microenvironment (TME) of breast and ovarian cancer. Striking changes in collagen architecture are associated with these epithelial cancers, and SHG can image these changes with great sensitivity and specificity with submicrometer resolution. This information has not historically been exploited by pathologists but has the potential to enhance diagnostic and prognostic capabilities. We summarize the utility of image processing tools that analyze fiber morphology in SHG images of breast and ovarian cancer in human tissues and animal models. We also describe methods that exploit the SHG physical underpinnings that are effective in delineating normal and malignant tissues. First we describe the use of polarization-resolved SHG that yields metrics related to macromolecular and supramolecular structures. The coherence and corresponding phase-matching process of SHG results in emission directionality (forward to backward), which is related to sub-resolution fibrillar assembly. These analyses are more general and more broadly applicable than purely morphology-based analyses; however, they are more computationally intensive. Intravital imaging techniques are also emerging that incorporate all of these quantitative analyses. Now, all these techniques can be coupled with rapidly advancing miniaturization of imaging systems to afford their use in clinical situations including enhancing pathology analysis and also in assisting in real-time surgical determination of tumor margins.
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Registered trials
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