ArticleInfection and immunity2015
Pathogenic Leptospira interrogans exoproteins are primarily involved in heterotrophic processes.
Article in Infection and immunity, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 46 citations in OpenAlex.
- Glutamine from plasma-like medium fuels nitrogen metabolism and influences proliferation in Leptospira interrogans.PLoS pathogens · 2026Article
- TheInfection and immunity · 2026Article
- Leptospira glyceraldehyde-3-phosphate dehydrogenase (LiGAPDH): a cell-surface plasminogen binding protein.FEMS microbiology letters · 2026Article
- Divergent Fates of Hardjo Leptospires: Early Transcriptomic Response ofTransboundary and emerging diseases · 2026Article
- Leucine-rich repeat proteins ofFrontiers in microbiology · 2024Article
- Leptospiral adhesins: from identification to future perspectives.Frontiers in microbiology · 2024Review
- Proteomic profile of naturally released extracellular vesicles secreted from Leptospira interrogans serovar Pomona in response to temperature and osmotic stresses.Scientific reports · 2023Article
- Leptospiral imelysin (LIC_10713) is secretory, immunogenic and binds to laminin, fibronectin, and collagen IV.Applied microbiology and biotechnology · 2023Article
- The structure ofFrontiers in immunology · 2023Article
- A New Recombinant Multiepitope Chimeric Protein ofTropical medicine and infectious disease · 2022Article
- Identifying Leptospira interrogans putative virulence factors with a yeast protein expression screen.Applied microbiology and biotechnology · 2022Article
- Extracellular Proteome Analysis Shows the Abundance of Histidine Kinase Sensor Protein, DNA Helicase, Putative Lipoprotein Containing Peptidase M75 Domain and Peptidase C39 Domain Protein inPathogens (Basel, Switzerland) · 2021Article
- Leptospiral Infection, Pathogenesis and Its Diagnosis-A Review.Pathogens (Basel, Switzerland) · 2021Review
- In Silico Structural and Functional Characterization of HtrA Proteins ofTropical medicine and infectious disease · 2020Article
- Characterization of a Novel Peptide from PathogenicPathogens (Basel, Switzerland) · 2020Article
- An extracellular Leptospira interrogans leucine-rich repeat protein binds human E- and VE-cadherins.Cellular microbiology · 2019Article
- vWA proteins of Leptospira interrogans induce hemorrhage in leptospirosis by competitive inhibition of vWF/GPIb-mediated platelet aggregation.EBioMedicine · 2018Article
- Article
- PathogenicOpen veterinary journal · 2018Review
- Virulence of the zoonotic agent of leptospirosis: still terra incognita?Nature reviews. Microbiology · 2017Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Leptospirosis is a life-threatening and emerging zoonotic disease with a worldwide annual occurrence of more than 1 million cases. Leptospirosis is caused by spirochetes belonging to the genus Leptospira. The mechanisms of disease manifestation in the host remain elusive, and the roles of leptospiral exoproteins in these processes have yet to be determined. Our aim in this study was to assess the composition and quantity of exoproteins of pathogenic Leptospira interrogans and to construe how these proteins contribute to disease pathogenesis. Label-free quantitative mass spectrometry of proteins obtained from Leptospira spirochetes cultured in vitro under conditions mimicking infection identified 325 exoproteins. The majority of these proteins are conserved in the nonpathogenic species Leptospira biflexa, and proteins involved in metabolism and energy-generating functions were overrepresented and displayed the highest relative abundance in culture supernatants. Conversely, proteins of unknown function, which represent the majority of pathogen-specific proteins (presumably involved in virulence mechanisms), were underrepresented. Characterization of various L. interrogans exoprotein mutants in the animal infection model revealed host mortality rates similar to those of hosts infected with wild-type L. interrogans. Collectively, these results indicate that pathogenic Leptospira exoproteins primarily function in heterotrophic processes (the processes by which organisms utilize organic substances as nutrient sources) to maintain the saprophytic lifestyle rather than the virulence of the bacteria. The underrepresentation of proteins homologous to known virulence factors, such as toxins and effectors in the exoproteome, also suggests that disease manifesting from Leptospira infection is likely caused by a combination of the primary and potentially moonlight functioning of exoproteins.
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