ArticleLab on a chip2015
Whole blood human neutrophil trafficking in a microfluidic model of infection and inflammation.
Article in Lab on a chip, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
39 citing papers in PubMed, 61 citations in OpenAlex.
- Modelling the innate immune system in microphysiological systems.Lab on a chip · 2024Review
- Passive redirection filters minimize red blood cell contamination during neutrophil chemotaxis assays using whole blood.Lab on a chip · 2023Article
- Advanced Microfluidic Vascularized Tissues as Platform for the Study of Human Diseases and Drug Development.Current neuropharmacology · 2023Article
- Integration of immune cells in organs-on-chips: a tutorial.Frontiers in bioengineering and biotechnology · 2023Review
- Article
- Optically Manipulated Neutrophils as Native MicrocraftsACS central science · 2022Article
- Joint-on-chip platforms: entering a new era of in vitro models for arthritis.Nature reviews. Rheumatology · 2022Review
- Modeling human T1D-associated autoimmune processes.Molecular metabolism · 2022Review
- Article
- Neutrophil dysfunction in cystic fibrosis.Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society · 2021Article
- Fully Automated, Sample-to-Answer Leukocyte Functional Assessment Platform for Continuous Sepsis Monitoring via Microliters of Blood.ACS sensors · 2021Article
- Critical Considerations for the Design of Multi-Organ Microphysiological Systems (MPS).Frontiers in cell and developmental biology · 2021Review
- A novel computer vision-based assessment of neutrophil chemotaxis in patients with severe infection.Clinical & translational immunology · 2021Article
- Microfluidic Systems to Study Neutrophil Forward and Reverse Migration.Frontiers in immunology · 2021Review
- Activation and Functional Priming of Blood Neutrophils in Non-Alcoholic Fatty Liver Disease Increases in Non-Alcoholic Steatohepatitis.Clinical and experimental gastroenterology · 2021Article
- Microphysiological Systems for Studying Cellular Crosstalk During the Neutrophil Response to Infection.Frontiers in immunology · 2021Review
- Immune Organs and Immune Cells on a Chip: An Overview of Biomedical Applications.Micromachines · 2020Review
- Differential Monocyte Actuation in a Three-Organ Functional Innate Immune System-on-a-Chip.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Article
- Semaphorin 3F signaling actively retains neutrophils at sites of inflammation.The Journal of clinical investigation · 2020Article
- Microfluidic devices for neutrophil chemotaxis studies.Journal of translational medicine · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
Abstract
Appropriate inflammatory responses to wounds and infections require adequate numbers of neutrophils arriving at injury sites. Both insufficient and excessive neutrophil recruitment can be detrimental, favouring systemic spread of microbes or triggering severe tissue damage. Despite its importance in health and disease, the trafficking of neutrophils through tissues remains difficult to control and the mechanisms regulating it are insufficiently understood. These mechanisms are also complex and difficult to isolate using traditional in vivo models. Here we designed a microfluidic model of tissue infection/inflammation, in which human neutrophils emerge from a droplet-size samples of whole blood and display bi-directional traffic between this and micro-chambers containing chemoattractant and microbe-like particles. Two geometrical barriers restrict the entrance of red blood cells from the blood to the micro-chambers and simulate the mechanical function of the endothelial barrier separating the cells in blood from cells in tissues. We found that in the presence of chemoattractant, the number of neutrophils departing the chambers by retrotaxis is in dynamic equilibrium with the neutrophils recruited by chemotaxis. We also found that in the presence of microbe-like particles, the number of neutrophils trapped in the chambers is proportional to the number of particles. Together, the dynamic equilibrium between migration, reversed-migration and trapping processes determine the optimal number of neutrophils at a site. These neutrophils are continuously refreshed and responsive to the number of microbes. Further studies using this infection-inflammation-on-a-chip-model could help study the processes of inflammation resolution. The new in vitro experimental tools may also eventually help testing new therapeutic strategies to limit neutrophil accumulation in tissues during chronic inflammation, without increasing the risk for infections.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.