Evidence map›Paper›PMID 25986989›Full record

ArticleBiochimica et biophysica acta2015

Single-cell analysis of mast cell degranulation induced by airway smooth muscle-secreted chemokines.

Benjamin M Manning, Audrey F Meyer, Sarah M Gruba, Christy L Haynes

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biochimica et biophysica acta, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.9field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 15 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Review
  5. Review
  6. The impact of vitamin D on asthmatic human airway smooth muscle.Expert review of respiratory medicine · 2016
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Benjamin M ManningDepartment of Chemistry, University of Minnesota, Minneapolis, MN 55455, USA.
Audrey F MeyerDepartment of Chemistry, University of Minnesota, Minneapolis, MN 55455, USA.
Sarah M GrubaDepartment of Chemistry, University of Minnesota, Minneapolis, MN 55455, USA.
Christy L HaynesDepartment of Chemistry, University of Minnesota, Minneapolis, MN 55455, USA. Electronic address: chaynes@umn.edu.
University of Minnesota · US

Funding

Chemistry-Biology Interface Training GrantT32GM008700 · NIGMS · UNIVERSITY OF MINNESOTA TWIN CITIES · PI DISTEFANO, MARK D · 1999 to 2018
$4.0M
IMMUNE SYSTEM-ON-A-CHIP FOR QUANTITATIVE ANALYSIS OF CELL INTERACTIONS DURING ALLDP2OD004258 · OD · UNIVERSITY OF MINNESOTA · PI HAYNES, CHRISTY L · 2008 to 2008
$2.3M
NIGMS NIH HHS T32 GM008700NIH HHS 1 DP2 OD004258-01NIH HHS DP2 OD004258
6 · The paper itself

Abstract

backgroundAsthma is a chronic inflammatory disease characterized by narrowed airways, bronchial hyper-responsiveness, mucus hyper-secretion, and airway remodeling. Mast cell (MC) infiltration into airway smooth muscle (ASM) is a defining feature of asthma, and ASM regulates the inflammatory response by secreting chemokines, including CXCL10 and CCL5. Single cell analysis offers a unique approach to study specific cellular signaling interactions within large and complex signaling networks such as the inflammatory microenvironment in asthma.

methodsCarbon-fiber microelectrode amperometry was used to study the effects of ASM-secreted chemokines on mouse peritoneal MC degranulation.

resultsMC degranulation in response to CXCL10 and CCL5 was monitored at the single cell level. Relative to IgE-mediated degranulation, CXCL10- and CCL5-stimulated MCs released a decreased amount of serotonin per granule with fewer release events per cell. Decreased serotonin release per granule was correlated with increased spike half-width and rise-time values.

conclusionsMCs are directly activated by ASM-associated chemokines. CXCL10 and CCL5 induce less robust MC degranulation compared to IgE- and A23187-stimulation. The kinetics of MC degranulation are signaling pathway-dependent, suggesting a biophysical mechanism of regulated degranulation that incorporates control over granule trafficking, transport, and docking machinery. GENERAL SIGNIFICANCE: The biophysical mechanisms, including variations in number of exocytotic release events, serotonin released per granule, and the membrane kinetics of exocytosis that underlie MC degranulation in response to CXCL10 and CCL5 were characterized at the single cell level. These findings clarify the function of ASM-derived chemokines as instigators of MC degranulation relative to classical mechanisms of MC stimulation.

Indexed as

Cell DegranulationAnimalsBronchiCarbonCarbon FiberCells, CulturedChemokine CCL5Chemokine CXCL10ChemokinesMast CellsMiceMice, Inbred C57BLMicroelectrodesMuscle, SmoothSerotoninSingle-Cell AnalysisCarbonCarbon FiberCcl5 protein, mouseChemokine CCL5Chemokine CXCL10ChemokinesCxcl10 protein, mouseSerotoninAsthmaCarbon-fiber microelectrode amperometryExocytosis

Identifiers

PMID25986989
PMCPMC4516622
OpenAlexW2171308890

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.