ArticlePloS one2015
Establishment of a Successive Markerless Mutation System in Haemophilus parasuis through Natural Transformation.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed.
- Research progress onFrontiers in veterinary science · 2025Review
- A marker-free genetic manipulation method for Glaesserella parasuis strains developed by alternately culturing transformants at 37°C and 30°C.BMC biotechnology · 2024Article
- PK-PD Modeling and Optimal Dosing Regimen of Acetylkitasamycin againstAntibiotics (Basel, Switzerland) · 2022Article
- The molecular diversity of transcriptional factor TfoX is a determinant in natural transformation inFrontiers in microbiology · 2022Article
- Article
- Exploration of Clinical Breakpoint of Danofloxacin forAntibiotics (Basel, Switzerland) · 2021Article
- Natural Transformation ofFrontiers in microbiology · 2021Article
- Phage Therapy: What Have We Learned?Viruses · 2018Review
- Basic Characterization of Natural Transformation in a Highly TransformableFrontiers in cellular and infection microbiology · 2018Article
- A streptomycin resistance marker inPeerJ · 2018Article
- Efficient construction ofCanadian journal of veterinary research = Revue canadienne de recherche veterinaire · 2016Article
- HtrA Is Important for Stress Resistance and Virulence in Haemophilus parasuis.Infection and immunity · 2016Article
- Complete Genome Sequence of Highly Virulent Haemophilus parasuis Serotype 11 Strain SC1401.Genome announcements · 2016Article
- A TolC-Like Protein ofFrontiers in microbiology · 2016Article
Corrections and comments
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Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Haemophilus parasuis, belonging to the family Pasteurellaceae, is the causative agent of Glässer's disease leading to serious economic losses. In this study, a successive markerless mutation system for H. parasuis using two sequential steps of natural transformation was developed. By the first homologous recombination, the target genes were replaced by a cassette carrying kanamycin resistance gene and sacB (which confers sensitivity to sucrose) gene using kanamycin selection, followed by the second reconstruction to remove the selection cassette, with application of sucrose to further screen unmarked mutants. To improve DNA transformation frequency, several parameters have been analyzed further in this work. With this method, two unmarked deletions in one strain have been generated successfully. It is demonstrated that this system can be employed to construct multi-gene scarless deletions, which is of great help for developing live attenuated vaccines for H. parasuis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.