ArticlePloS one2015
Combining Next Generation Sequencing with Bulked Segregant Analysis to Fine Map a Stem Moisture Locus in Sorghum (Sorghum bicolor L. Moench).
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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29 citing papers in PubMed, 45 citations in OpenAlex.
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- Identification of salt stress-tolerant candidate genes in the BCFrontiers in plant science · 2023Article
- Construction of a high-density genetic linkage map and QTL mapping for bioenergy-related traits in sweet sorghum [Frontiers in plant science · 2023Article
- Genomic resources in plant breeding for sustainable agriculture.Journal of plant physiology · 2021Review
- Genetic Analysis of Stem Diameter and Water Contents To Improve Sorghum Bioenergy Efficiency.G3 (Bethesda, Md.) · 2020Article
- Identification of a Major QTL and Candidate Gene Analysis of Salt Tolerance at the Bud Burst Stage in Rice (Oryza sativa L.) Using QTL-Seq and RNA-Seq.Rice (New York, N.Y.) · 2020Article
- Identification of candidate tolerance genes to low-temperature during maize germination by GWAS and RNA-seqapproaches.BMC plant biology · 2020Article
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- Development of KASP Markers and Identification of a QTL Underlying Powdery Mildew Resistance in Melon (Frontiers in plant science · 2020Article
- Advances in Molecular Genetics and Genomics of African Rice (Plants (Basel, Switzerland) · 2019Review
- Mapping Cucumber Vein Yellowing Virus Resistance in Cucumber (Frontiers in plant science · 2019Article
- Sorghum stem aerenchyma formation is regulated byPlant direct · 2018Article
- Sweet Sorghum Originated through Selection ofThe Plant cell · 2018Article
- Transcriptional switch for programmed cell death in pith parenchyma of sorghum stems.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Fine mapping and identification of a novel locus qGL12.2 control grain length in wild rice (Oryza rufipogon Griff.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2018Article
- Role of genomics in promoting the utilization of plant genetic resources in genebanks.Briefings in functional genomics · 2018Review
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sorghum is one of the most promising bioenergy crops. Stem juice yield, together with stem sugar concentration, determines sugar yield in sweet sorghum. Bulked segregant analysis (BSA) is a gene mapping technique for identifying genomic regions containing genetic loci affecting a trait of interest that when combined with deep sequencing could effectively accelerate the gene mapping process. In this study, a dry stem sorghum landrace was characterized and the stem water controlling locus, qSW6, was fine mapped using QTL analysis and the combined BSA and deep sequencing technologies. Results showed that: (i) In sorghum variety Jiliang 2, stem water content was around 80% before flowering stage. It dropped to 75% during grain filling with little difference between different internodes. In landrace G21, stem water content keeps dropping after the flag leaf stage. The drop from 71% at flowering time progressed to 60% at grain filling time. Large differences exist between different internodes with the lowest (51%) at the 7th and 8th internodes at dough stage. (ii) A quantitative trait locus (QTL) controlling stem water content mapped on chromosome 6 between SSR markers Ch6-2 and gpsb069 explained about 34.7-56.9% of the phenotypic variation for the 5th to 10th internodes, respectively. (iii) BSA and deep sequencing analysis narrowed the associated region to 339 kb containing 38 putative genes. The results could help reveal molecular mechanisms underlying juice yield of sorghum and thus to improve total sugar yield.
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