ArticleFrontiers in plant science2015
The bZIP transcription factor HY5 interacts with the promoter of the monoterpene synthase gene QH6 in modulating its rhythmic expression.
Article in Frontiers in plant science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 57 citations in OpenAlex.
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- PfERF106, a novel key transcription factor regulating the biosynthesis of floral terpenoids in Primula forbesii Franch.BMC plant biology · 2024Article
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- Functions of Representative Terpenoids and Their Biosynthesis Mechanisms in Medicinal Plants.Biomolecules · 2023Review
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- Insights into the physiological, molecular, and genetic regulators of albinism inFrontiers in genetics · 2023Article
- Comparative transcriptome analysis revealed the molecular mechanism of the effect of light intensity on the accumulation of rhynchophylline and isorhynchophylline inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- Terpene synthases in cucumber (Cucumis sativus) and their contribution to herbivore-induced volatile terpenoid emission.The New phytologist · 2022Article
- Genome-wide identification and expression analysis of the bZIP transcription factors, and functional analysis in response to drought and cold stresses in pear (Pyrus breschneideri).BMC plant biology · 2021Article
- Transcriptome Analysis of Light-Regulated Monoterpenes Biosynthesis in Leaves ofPlants (Basel, Switzerland) · 2021Article
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- Effects of Light on Secondary Metabolite Biosynthesis in Medicinal Plants.Frontiers in plant science · 2021Review
- Transcriptional Factors Regulate Plant Stress Responses through Mediating Secondary Metabolism.Genes · 2020Review
- Transcriptomic data-driven discovery of global regulatory features of rice seeds developing under heat stress.Computational and structural biotechnology journal · 2020Article
- Transcriptomic Analysis of the Candidate Genes Related to Aroma Formation inMolecules (Basel, Switzerland) · 2018Article
- Diurnal regulation of the floral scent emission by light and circadian rhythm in the Phalaenopsis orchids.Botanical studies · 2017Article
- Drawing Links from Transcriptome to Metabolites: The Evolution of Aroma in the Ripening Berry of Moscato Bianco (Frontiers in plant science · 2017Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Artemisia annua L. β-pinene synthase QH6 was previously determined to be circadian-regulated at the transcriptional level, showing a rhythmic fluctuation of steady-state transcript abundances. Here we isolated both the genomic sequence and upstream promoter region of QH6. Different regulatory elements, such as G-box (TGACACGTGGCA, -421 bp from the translation initiation site) which might have effects on rhythmic gene expression, were found. Using the yeast one-hybrid and electrophoretic mobility shift assay (EMSA), we confirmed that the bZIP transcription factor HY5 binds to this motif of QH6. Studies with promoter truncations before and after this motif suggested that this G-box was important for the diurnal fluctuation of the transgenic β-glucuronidase gene (GUS) transcript abundance in Arabidopsis thaliana. GUS gene driven by the promoter region immediately after G-box showed an arrhythmic expression in both light/dark (LD) and constant dark (DD) conditions, whereas the control with G-box retained its fluctuation in both LD and DD. We further transformed A. thaliana with the luciferase gene (LUC) driven by an 1400 bp fragment upstream QH6 with its G-box intact or mutated, respectively. The luciferase activity assay showed that a peak in the early morning disappeared in the mutant. Gene expression analysis also demonstrated that the rhythmic expression of LUC was abolished in the hy5-1 mutant.
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