ArticleFrontiers in microbiology2015
Distinct summer and winter bacterial communities in the active layer of Svalbard permafrost revealed by DNA- and RNA-based analyses.
Article in Frontiers in microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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42 citing papers in PubMed, 124 citations in OpenAlex.
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- Exploring bacterial diversity in Arctic fjord sediments: a 16S rRNA-based metabarcoding portrait.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Seasonality in land-ocean connectivity and local processes control sediment bacterial community structure and function in a High Arctic tidal flat.FEMS microbiology ecology · 2024Article
- Warming effects on grassland soil microbial communities are amplified in cool months.The ISME journal · 2024Article
- PRA Melting-ICE Project: Svalbard 2022 Expeditions Report.Open research Europe · 2024Article
- Clearing the air: unraveling past and guiding future research in atmospheric chemosynthesis.Microbiology and molecular biology reviews : MMBR · 2023Review
- Distinct taxonomic and functional profiles of high Arctic and alpine permafrost-affected soil microbiomes.Environmental microbiome · 2023Article
- RNA-based amplicon sequencing is ineffective in measuring metabolic activity in environmental microbial communities.Microbiome · 2023Article
- Functional microbial ecology in arctic soils: the need for a year-round perspective.FEMS microbiology ecology · 2022Article
- Soil Microbial Communities Associated with Three Arctic Plants in Different Local Environments in Ny-Ålesund, Svalbard.Journal of microbiology and biotechnology · 2022Article
- The activity and functions of soil microbial communities in the Finnish sub-Arctic vary across vegetation types.FEMS microbiology ecology · 2022Article
- Article
- Total nitrogen influence bacterial community structure of active layer permafrost across summer and winter seasons in Ny-Ålesund, Svalbard.World journal of microbiology & biotechnology · 2022Article
- Microbial Community Changes in 26,500-Year-Old Thawing Permafrost.Frontiers in microbiology · 2022Article
- Seasonality of Glacial Snow and Ice Microbial Communities.Frontiers in microbiology · 2022Article
- Impact of River Channel Lateral Migration on Microbial Communities across a Discontinuous Permafrost Floodplain.Applied and environmental microbiology · 2021Article
- Microbial characterisation and Cold-Adapted Predicted Protein (CAPP) database construction from the active layer of Greenland's permafrost.FEMS microbiology ecology · 2021Article
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Authors and funding
9 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The active layer of soil overlaying permafrost in the Arctic is subjected to dramatic annual changes in temperature and soil chemistry, which likely affect bacterial activity and community structure. We studied seasonal variations in the bacterial community of active layer soil from Svalbard (78°N) by co-extracting DNA and RNA from 12 soil cores collected monthly over a year. PCR amplicons of 16S rRNA genes (DNA) and reverse transcribed transcripts (cDNA) were quantified and sequenced to test for the effect of low winter temperature and seasonal variation in concentration of easily degradable organic matter on the bacterial communities. The copy number of 16S rRNA genes and transcripts revealed no distinct seasonal changes indicating potential bacterial activity during winter despite soil temperatures well below -10°C. Multivariate statistical analysis of the bacterial diversity data (DNA and cDNA libraries) revealed a season-based clustering of the samples, and, e.g., the relative abundance of potentially active Cyanobacteria peaked in June and Alphaproteobacteria increased over the summer and then declined from October to November. The structure of the bulk (DNA-based) community was significantly correlated with pH and dissolved organic carbon, while the potentially active (RNA-based) community structure was not significantly correlated with any of the measured soil parameters. A large fraction of the 16S rRNA transcripts was assigned to nitrogen-fixing bacteria (up to 24% in June) and phototrophic organisms (up to 48% in June) illustrating the potential importance of nitrogen fixation in otherwise nitrogen poor Arctic ecosystems and of phototrophic bacterial activity on the soil surface.
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