ArticleScientific reports2015
High-Throughput Microfluidic Platform for 3D Cultures of Mesenchymal Stem Cells, Towards Engineering Developmental Processes.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 85 citations in OpenAlex.
- A Microwell Array Embedded Microfluidic Gradient Platform for Drug Screening on Tumor Spheroids.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- A vascularized microfluidic model of the osteochondral unit for modeling inflammatory response and therapeutic screening.Lab on a chip · 2025Article
- Microfluidic Systems for Neural Cell Studies.Bioengineering (Basel, Switzerland) · 2023Review
- Recent development in multizonal scaffolds for osteochondral regeneration.Bioactive materials · 2023Review
- Current Status of Mesenchymal Stem/Stromal Cells for Treatment of Neurological Diseases.Frontiers in molecular neuroscience · 2022Review
- Specificity of 3D MSC Spheroids Microenvironment: Impact on MSC Behavior and Properties.Stem cell reviews and reports · 2020Review
- Mesenchymal Stem Cells as a Promising Cell Source for Integration in Novel In Vitro Models.Biomolecules · 2020Review
- Wnt3a and wnt5a as Potential Chondrogenic Stimulators for Nucleus Pulposus Cell Induction: A Comprehensive Review.Neurospine · 2020Article
- Microfluidic three-dimensional cell culture of stem cells for high-throughput analysis.World journal of stem cells · 2019Review
- Modular Pressure and Flow Rate-Balanced Microfluidic Serial Dilution Networks for Miniaturised Point-of-Care Diagnostic Platforms.Sensors (Basel, Switzerland) · 2019Article
- A Microfluidic Spheroid Culture Device with a Concentration Gradient Generator for High-Throughput Screening of Drug Efficacy.Molecules (Basel, Switzerland) · 2018Article
- A Fluidic Culture Platform for Spatially Patterned Cell Growth, Differentiation, and Cocultures.Tissue engineering. Part A · 2018Article
- Towards Three-Dimensional Dynamic Regulation and In Situ Characterization of Single Stem Cell Phenotype Using Microfluidics.Molecular biotechnology · 2018Review
- Stem cells technology: a powerful tool behind new brain treatments.Drug delivery and translational research · 2018Review
- Effects of three-dimensional spheroid culture on equine mesenchymal stem cell plasticity.Veterinary research communications · 2018Article
- Developmentally inspired programming of adult human mesenchymal stromal cells toward stable chondrogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Super-resolution for asymmetric resolution of FIB-SEM 3D imaging using AI with deep learning.Scientific reports · 2018Article
- High-throughput approaches for screening and analysis of cell behaviors.Biomaterials · 2018Review
- Translational Application of Microfluidics and Bioprinting for Stem Cell-Based Cartilage Repair.Stem cells international · 2018Review
- Honing Cell and Tissue Culture Conditions for Bone and Cartilage Tissue Engineering.Cold Spring Harbor perspectives in medicine · 2017Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The development of in vitro models to screen the effect of different concentrations, combinations and temporal sequences of morpho-regulatory factors on stem/progenitor cells is crucial to investigate and possibly recapitulate developmental processes with adult cells. Here, we designed and validated a microfluidic platform to (i) allow cellular condensation, (ii) culture 3D micromasses of human bone marrow-derived mesenchymal stromal cells (hBM-MSCs) under continuous flow perfusion, and (ii) deliver defined concentrations of morphogens to specific culture units. Condensation of hBM-MSCs was obtained within 3 hours, generating micromasses in uniform sizes (56.2 ± 3.9 μm). As compared to traditional macromass pellet cultures, exposure to morphogens involved in the first phases of embryonic limb development (i.e. Wnt and FGF pathways) yielded more uniform cell response throughout the 3D structures of perfused micromasses (PMMs), and a 34-fold higher percentage of proliferating cells at day 7. The use of a logarithmic serial dilution generator allowed to identify an unexpected concentration of TGFβ3 (0.1 ng/ml) permissive to hBM-MSCs proliferation and inductive to chondrogenesis. This proof-of-principle study supports the described microfluidic system as a tool to investigate processes involved in mesenchymal progenitor cells differentiation, towards a 'developmental engineering' approach for skeletal tissue regeneration.
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Registered trials
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