ArticleMolecular microbiology2015
Repression by H-NS of genes required for the biosynthesis of the Vibrio cholerae biofilm matrix is modulated by the second messenger cyclic diguanylic acid.
Article in Molecular microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 51 citations in OpenAlex.
- Environmental regulation of virulence in Vibrio cholerae: integrating multi-omics, predictive models, and comparative insights across emerging Vibrio species.Gut pathogens · 2026Review
- Molecular Adaptations of Ferric Uptake Regulator (Fur) Proteins Drive Iron Homeostasis and Pathogenesis in Vibrio Spp.Current microbiology · 2026Review
- Negative feedback of cyclic di-GMP levels optimizes switching between sessile and motile lifestyles inbioRxiv : the preprint server for biology · 2024Article
- Increased intracellular persulfide levels attenuate HlyU-mediated hemolysin transcriptional activation inbioRxiv : the preprint server for biology · 2023Article
- New Insights into Vibrio cholerae Biofilms from Molecular Biophysics to Microbial Ecology.Advances in experimental medicine and biology · 2023Article
- Conserved FimK Truncation Coincides with Increased Expression of Type 3 Fimbriae and Cultured Bladder Epithelial Cell Association in Klebsiella quasipneumoniae.Journal of bacteriology · 2022Article
- Genome-wide mapping of Vibrio cholerae VpsT binding identifies a mechanism for c-di-GMP homeostasis.Nucleic acids research · 2022Article
- A phage-encoded nucleoid associated protein compacts both host and phage DNA and derepresses H-NS silencing.Nucleic acids research · 2021Article
- TsrA Regulates Virulence and Intestinal Colonization inmSphere · 2020Article
- Transcriptional Silencing by TsrA in the Evolution of Pathogenic Vibrio cholerae Biotypes.mBio · 2020Article
- Motility of Vibrio spp.: regulation and controlling strategies.Applied microbiology and biotechnology · 2020Review
- VpsR Directly Activates Transcription of Multiple Biofilm Genes in Vibrio cholerae.Journal of bacteriology · 2020Article
- Article
- Reciprocal c-di-GMP signaling: Incomplete flagellum biogenesis triggers c-di-GMP signaling pathways that promote biofilm formation.PLoS genetics · 2020Article
- Global H-NS counter-silencing by LuxR activates quorum sensing gene expression.Nucleic acids research · 2020Article
- Fur RepressesFrontiers in microbiology · 2020Article
- Article
- Cyclic di-GMP Increases Catalase Production and Hydrogen Peroxide Tolerance inApplied and environmental microbiology · 2019Article
- VpsR and cyclic di-GMP together drive transcription initiation to activate biofilm formation in Vibrio cholerae.Nucleic acids research · 2018Article
- Cyclic di-GMP Positively Regulates DNA Repair in Vibrio cholerae.Journal of bacteriology · 2018Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Expression of Vibrio cholerae genes required for the biosynthesis of exopolysacchide (vps) and protein (rbm) components of the biofilm matrix is enhanced by cyclic diguanylate (c-di-GMP). In a previous study, we reported that the histone-like nucleoid structuring (H-NS) protein represses the transcription of vpsA, vpsL and vpsT. Here we demonstrate that the regulator VpsT can disrupt repressive H-NS nucleoprotein complexes at the vpsA and vpsL promoters in the presence of c-di-GMP, while H-NS could disrupt the VpsT-promoter complexes in the absence of c-di-GMP. Chromatin immunoprecipitation-Seq showed a remarkable trend for H-NS to cluster at loci involved in biofilm development such as the rbmABCDEF genes. We show that the antagonistic relationship between VpsT and H-NS regulates the expression of the rbmABCDEF cluster. Epistasis analysis demonstrated that VpsT functions as an antirepressor at the rbmA/F, vpsU and vpsA/L promoters. Deletion of vpsT increased H-NS occupancy at these promoters while increasing the c-di-GMP pool had the opposite effect and included the vpsT promoter. The negative effect of c-di-GMP on H-NS occupancy at the vpsT promoter required the regulator VpsR. These results demonstrate that c-di-GMP activates the transcription of genes required for the biosynthesis of the biofilm matrix by triggering a coordinated VpsR- and VpsT-dependent H-NS antirepression cascade.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.