ArticleBiochemistry2015
Protein Synthesis with Ribosomes Selected for the Incorporation of β-Amino Acids.
Article in Biochemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 71 citations in OpenAlex.
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- RNA language models predict mutations that improve RNA function.bioRxiv : the preprint server for biology · 2024Article
- Fine-tuning the tRNA anticodon arm for multiple/consecutive incorporations of β-amino acids and analogs.Nucleic acids research · 2024Article
- Engineered mRNA-ribosome fusions for facile biosynthesis of selenoproteins.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Elongation Factor P Modulates the Incorporation of Structurally Diverse Noncanonical Amino Acids intoJournal of the American Chemical Society · 2023Article
- Cell-free Biosynthesis of Peptidomimetics.Biotechnology and bioprocess engineering : BBE · 2023Review
- Modular Synthesis of Unnatural Peptides via Rh(III)-Catalyzed Diastereoselective Three-Component Carboamidation Reaction.Journal of the American Chemical Society · 2023Article
- Ribosome-mediated biosynthesis of pyridazinone oligomers in vitro.Nature communications · 2022Article
- In Vitro Selection of Macrocyclic α/βJournal of the American Chemical Society · 2022Article
- Three-dimensional structure-guided evolution of a ribosome with tethered subunits.Nature chemical biology · 2022Article
- Expansion of the Genetic Code Through the Use of Modified Bacterial Ribosomes.Journal of molecular biology · 2022Review
- Redirecting RiPP Biosynthetic Enzymes to Proteins and Backbone-Modified Substrates.ACS central science · 2022Article
- Synthetic auxotrophy remains stable after continuous evolution and in coculture with mammalian cells.Science advances · 2021Article
- Facilitated synthesis of proteins containing modified dipeptides.Bioorganic & medicinal chemistry · 2021Article
- Reprogramming the genetic code.Nature reviews. Genetics · 2021Review
- Mechanistic studies of non-canonical amino acid mutagenesis.Methods in enzymology · 2021Article
- Ribosomal synthesis and de novo discovery of bioactive foldamer peptides containing cyclic β-amino acids.Nature chemistry · 2020Article
- Ribosome-mediated polymerization of long chain carbon and cyclic amino acids into peptides in vitro.Nature communications · 2020Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
In an earlier study, β³-puromycin was used for the selection of modified ribosomes, which were utilized for the incorporation of five different β-amino acids into Escherichia coli dihydrofolate reductase (DHFR). The selected ribosomes were able to incorporate structurally disparate β-amino acids into DHFR, in spite of the use of a single puromycin for the selection of the individual clones. In this study, we examine the extent to which the structure of the β³-puromycin employed for ribosome selection influences the regio- and stereochemical preferences of the modified ribosomes during protein synthesis; the mechanistic probe was a single suppressor tRNA(CUA) activated with each of four methyl-β-alanine isomers (1-4). The modified ribosomes were found to incorporate each of the four isomeric methyl-β-alanines into DHFR but exhibited a preference for incorporation of 3(S)-methyl-β-alanine (β-mAla; 4), i.e., the isomer having the same regio- and stereochemistry as the O-methylated β-tyrosine moiety of β³-puromycin. Also conducted were a selection of clones that are responsive to β²-puromycin and a demonstration of reversal of the regio- and stereochemical preferences of these clones during protein synthesis. These results were incorporated into a structural model of the modified regions of 23S rRNA, which included in silico prediction of a H-bonding network. Finally, it was demonstrated that incorporation of 3(S)-methyl-β-alanine (β-mAla; 4) into a short α-helical region of the nucleic acid binding domain of hnRNP LL significantly stabilized the helix without affecting its DNA binding properties.
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