ArticleMalaria journal2015
Field deployment of loop-mediated isothermal amplification for centralized mass-screening of asymptomatic malaria in Zanzibar: a pre-elimination setting.
Article in Malaria journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 1 of them a synthesis that pooled it.
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Who cites it
34 citing papers in PubMed, 1 synthesis or guideline pooled it, 50 citations in OpenAlex.
- Targeted Testing and Treatment To Reduce Human Malaria Transmission in High-Risk Populations: A Systematic Review.The American journal of tropical medicine and hygiene · 2024Pooled it
- An affordable detection system based on RT-LAMP and DNA-nanoprobes for avian metapneumovirus.Applied microbiology and biotechnology · 2024Article
- First field and laboratory evaluation of LAMP assay for malaria diagnosis in Cubal, Angola.Parasites & vectors · 2023Article
- Performance of the procedure for ultra-rapid extraction and loop-mediated isothermal amplification (PURE-LAMP) method to detect malaria in Haiti.Infectious diseases of poverty · 2023Article
- Spatiotemporal dynamics of malaria in Zanzibar, 2015-2020.BMJ global health · 2023Article
- Assessing afebrile malaria and bed-net use in a high-burden region of India: Findings from multiple rounds of mass screening.PloS one · 2023Article
- MINI: A high-throughput point-of-care device for performing hundreds of nucleic acid tests per day.Biosensors & bioelectronics · 2022Article
- Usefulness of a commercial LAMP assay for detection of malaria infection, including Plasmodium knowlesi cases, in returning travelers in Spain.BMC research notes · 2022Article
- Accuracy of a rapid diagnosis test, microscopy and loop-mediated isothermal amplification in the detection of asymptomatic Plasmodium infections in Korhogo, Northern Côte d'Ivoire.Malaria journal · 2022Article
- Conditional trust: Community perceptions of drone use in malaria control in Zanzibar.Technology in society · 2022Article
- Sample-to-Answer Microfluidic Nucleic Acid Testing (NAT) on Lab-on-a-Disc for Malaria Detection at Point of Need.Methods in molecular biology (Clifton, N.J.) · 2022Article
- An Ultracompact Real-Time Fluorescence Loop-Mediated Isothermal Amplification (LAMP) Analyzer.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Performance and Application of Commercially Available Loop-Mediated Isothermal Amplification (LAMP) Kits in Malaria Endemic and Non-Endemic Settings.Diagnostics (Basel, Switzerland) · 2021Review
- Article
- Detection ofThe American journal of tropical medicine and hygiene · 2019Article
- From high to low malaria transmission in Zanzibar-challenges and opportunities to achieve elimination.BMC medicine · 2019Article
- Sample-to-answer palm-sized nucleic acid testing device towards low-cost malaria mass screening.Biosensors & bioelectronics · 2018Article
- Diagnostic accuracy of loop-mediated isothermal amplification (LAMP) for screening malaria in peripheral and placental blood samples from pregnant women in Colombia.Malaria journal · 2018Article
- An innovative diagnostic technology for the codon mutation C580Y in kelch13 of Plasmodium falciparum with MinION nanopore sequencer.Malaria journal · 2018Article
- Emerging implications of policies on malaria treatment: genetic changes in theBMJ global health · 2018Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 4 countries.
Funding
Abstract
backgroundMolecular tools for detection of low-density asymptomatic Plasmodium infections are needed in malaria elimination efforts. This study reports results from the hitherto largest implementation of loop-mediated isothermal amplification (LAMP) for centralized mass screening of asymptomatic malaria in Zanzibar.
methodsHealthy individuals present and willing to participate in randomly selected households in 60 villages throughout Zanzibar were screened for malaria by rapid diagnostic tests (RDT). In 50% of the study households, participants were asked to provide 60 μL of finger-prick blood for additional LAMP screening. LAMP was conducted in two centralized laboratories in Zanzibar, by trained technicians with limited or no previous experience of molecular methods. The LAMP assay was performed with Loopamp(TM) MALARIA Pan/Pf Detection Kit (Eiken Chemical Company, Japan). Samples positive for Plasmodium genus (Pan)-LAMP were re-tested using Plasmodium falciparum-specific LAMP kits.
resultsPaired RDT and LAMP samples were available from 3983 individuals. The prevalence of asymptomatic malaria was 0.5% (CI 95% 0.1-0.8) and 1.6% (CI 95% 1.1-2.2) by RDT and Pan-LAMP, respectively. LAMP detected 3.4 (CI 95% 2.2-5.2) times more Plasmodium positive samples than RDT. DNA contamination was experienced, but solved by repetitive decontamination of all equipment and reagents.
conclusionsLAMP is a simple and sensitive molecular tool, and has potential in active surveillance and mass-screening programmes for detection of low-density asymptomatic malaria in pre-elimination settings. However, in order to deploy LAMP more effectively in field settings, protocols may need to be adapted for processing larger numbers of samples. A higher throughput, affordable closed system would be ideal to avoid contamination.
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